Borut Kovacic1, Mojca Cizek Sajko, Veljko Vlaisavljević. 1. Department of Reproductive Medicine and Gynecologic Endocrinology, University Clinical Centre Maribor, Maribor, Slovenia. borut.kov@ukc-mb.si
Abstract
OBJECTIVE: To assess whether the embryo cultivation at different oxygen tensions had any effect on intracytoplasmic sperm injection (ICSI) outcome. DESIGN: Prospective randomized trial. SETTING: University clinical center. PATIENT(S): Women undergoing ICSI (n = 647). INTERVENTION(S): Culturing of embryos, either at 6% CO(2), 5% O(2), 89% N(2), or at 6% CO(2) in air. MAIN OUTCOME MEASURE(S): The primary outcome was ongoing pregnancy rates (PR). SECONDARY OUTCOMES: cumulative PRs, implantation, and embryo quality, for both treatment groups and clinical outcomes for subgroups (optimal cycles, poor responders, older women). RESULT(S): Although low oxygen resulted in a higher proportion of good day 2 embryos (0.547 +/- 0.021 vs. 0.39 +/- 0.019) and optimal blastocysts (0.162 +/- 0.199 vs. 0.083 +/- 0.128), the ongoing PRs (31.6% vs. 27.1%) and implantation rates (28.8% vs. 25.2%) were similar in both oxygen groups. Low oxygen caused a higher cumulative PR (38% vs. 28.3%) in the main group and a higher PR in the poor responder subgroup (23% vs. 9.8%) with embryo transfers performed mostly on day 3. CONCLUSION(S): The use of reduced oxygen in IVF is reasonable, irrespective of the duration of embryo culture. It improves embryo development and cumulative PR and is also recommended in poor responding cycles. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
RCT Entities:
OBJECTIVE: To assess whether the embryo cultivation at different oxygen tensions had any effect on intracytoplasmic sperm injection (ICSI) outcome. DESIGN: Prospective randomized trial. SETTING: University clinical center. PATIENT(S): Women undergoing ICSI (n = 647). INTERVENTION(S): Culturing of embryos, either at 6% CO(2), 5% O(2), 89% N(2), or at 6% CO(2) in air. MAIN OUTCOME MEASURE(S): The primary outcome was ongoing pregnancy rates (PR). SECONDARY OUTCOMES: cumulative PRs, implantation, and embryo quality, for both treatment groups and clinical outcomes for subgroups (optimal cycles, poor responders, older women). RESULT(S): Although low oxygen resulted in a higher proportion of good day 2 embryos (0.547 +/- 0.021 vs. 0.39 +/- 0.019) and optimal blastocysts (0.162 +/- 0.199 vs. 0.083 +/- 0.128), the ongoing PRs (31.6% vs. 27.1%) and implantation rates (28.8% vs. 25.2%) were similar in both oxygen groups. Low oxygen caused a higher cumulative PR (38% vs. 28.3%) in the main group and a higher PR in the poor responder subgroup (23% vs. 9.8%) with embryo transfers performed mostly on day 3. CONCLUSION(S): The use of reduced oxygen in IVF is reasonable, irrespective of the duration of embryo culture. It improves embryo development and cumulative PR and is also recommended in poor responding cycles. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Authors: David B Gomes Sobrinho; Joao Batista A Oliveira; Claudia G Petersen; Ana L Mauri; Liliane F I Silva; Fabiana C Massaro; Ricardo L R Baruffi; Mario Cavagna; José G Franco Journal: Reprod Biol Endocrinol Date: 2011-11-01 Impact factor: 5.211
Authors: Alexandra Meuter; Lisa-Marlen Rogmann; Boris J Winterhoff; Tamar Tchkonia; James L Kirkland; Dean E Morbeck Journal: J Assist Reprod Genet Date: 2014-08-10 Impact factor: 3.412