Literature DB >> 19405035

High-throughput generation of tagged stable cell lines for proteomic analysis.

Jorge Z Torres1, Julie J Miller, Peter K Jackson.   

Abstract

We present an optimized system for rapid generation of localization and affinity purification-tagged mammalian stable cell lines that facilitates complex purification and interacting protein identification. The improved components of this method, including the flexibility of inducible expression, circumvent issues associated with toxicity, clonal selection, sample yields and time to data acquisition. We have applied this method to the study of cell-cycle regulators and novel microtubule-associated proteins.

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Year:  2009        PMID： 19405035      PMCID： PMC4785821          DOI： 10.1002/pmic.200800873

Source DB:  PubMed          Journal:  Proteomics        ISSN： 1615-9853            Impact factor:   3.984


  14 in total

1.  DNA cloning using in vitro site-specific recombination.

Authors:  J L Hartley; G F Temple; M A Brasch
Journal:  Genome Res       Date:  2000-11       Impact factor: 9.043

Review 2.  The tandem affinity purification (TAP) method: a general procedure of protein complex purification.

Authors:  O Puig; F Caspary; G Rigaut; B Rutz; E Bouveret; E Bragado-Nilsson; M Wilm; B Séraphin
Journal:  Methods       Date:  2001-07       Impact factor: 3.608

3.  An efficient protein complex purification method for functional proteomics in higher eukaryotes.

Authors:  Daniel Forler; Thomas Köcher; Michaela Rode; Mark Gentzel; Elisa Izaurralde; Matthias Wilm
Journal:  Nat Biotechnol       Date:  2002-12-16       Impact factor: 54.908

Review 4.  Dynamic, structural, and regulatory aspects of lambda site-specific recombination.

Authors:  A Landy
Journal:  Annu Rev Biochem       Date:  1989       Impact factor: 23.643

Review 5.  ORFeome cloning and systems biology: standardized mass production of the parts from the parts-list.

Authors:  Michael A Brasch; James L Hartley; Marc Vidal
Journal:  Genome Res       Date:  2004-10       Impact factor: 9.043

6.  Microtubules cut and run.

Authors:  Peter W Baas; Arzu Karabay; Liang Qiang
Journal:  Trends Cell Biol       Date:  2005-10       Impact factor: 20.808

7.  A combined approach for the localization and tandem affinity purification of protein complexes from metazoans.

Authors:  Iain M Cheeseman; Arshad Desai
Journal:  Sci STKE       Date:  2005-01-11

8.  Katanin, the microtubule-severing ATPase, is concentrated at centrosomes.

Authors:  F J McNally; K Okawa; A Iwamatsu; R D Vale
Journal:  J Cell Sci       Date:  1996-03       Impact factor: 5.285

9.  Katanin controls mitotic and meiotic spindle length.

Authors:  Karen McNally; Anjon Audhya; Karen Oegema; Francis J McNally
Journal:  J Cell Biol       Date:  2006-12-18       Impact factor: 10.539

10.  hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes.

Authors:  Philippe Lamesch; Ning Li; Stuart Milstein; Changyu Fan; Tong Hao; Gabor Szabo; Zhenjun Hu; Kavitha Venkatesan; Graeme Bethel; Paul Martin; Jane Rogers; Stephanie Lawlor; Stuart McLaren; Amélie Dricot; Heather Borick; Michael E Cusick; Jean Vandenhaute; Ian Dunham; David E Hill; Marc Vidal
Journal:  Genomics       Date:  2007-01-05       Impact factor: 5.736
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  67 in total

1.  Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome.

Authors:  Christopher J Westlake; Lisa M Baye; Maxence V Nachury; Kevin J Wright; Karen E Ervin; Lilian Phu; Cecile Chalouni; John S Beck; Donald S Kirkpatrick; Diane C Slusarski; Val C Sheffield; Richard H Scheller; Peter K Jackson
Journal:  Proc Natl Acad Sci U S A       Date:  2011-01-27       Impact factor: 11.205

2.  Optimization of Isothiazolo[4,3- b]pyridine-Based Inhibitors of Cyclin G Associated Kinase (GAK) with Broad-Spectrum Antiviral Activity.

Authors:  Szu-Yuan Pu; Randy Wouters; Stanford Schor; Jef Rozenski; Rina Barouch-Bentov; Laura I Prugar; Cecilia M O'Brien; Jennifer M Brannan; John M Dye; Piet Herdewijn; Steven De Jonghe; Shirit Einav
Journal:  J Med Chem       Date:  2018-07-16       Impact factor: 7.446

3.  Dependence of tumor cell lines and patient-derived tumors on the NAD salvage pathway renders them sensitive to NAMPT inhibition with GNE-618.

Authors:  Yang Xiao; Kristi Elkins; Jenni K Durieux; Leslie Lee; Jason Oeh; Lulu X Yang; Xiaorong Liang; Chris DelNagro; Jarrod Tremayne; Mandy Kwong; Bianca M Liederer; Peter K Jackson; Lisa D Belmont; Deepak Sampath; Thomas O'Brien
Journal:  Neoplasia       Date:  2013-10       Impact factor: 5.715

4.  Drosophila katanin is a microtubule depolymerase that regulates cortical-microtubule plus-end interactions and cell migration.

Authors:  Dong Zhang; Kyle D Grode; Shannon F Stewman; Juan Daniel Diaz-Valencia; Emily Liebling; Uttama Rath; Tania Riera; Joshua D Currie; Daniel W Buster; Ana B Asenjo; Hernando J Sosa; Jennifer L Ross; Ao Ma; Stephen L Rogers; David J Sharp
Journal:  Nat Cell Biol       Date:  2011-03-06       Impact factor: 28.824

5.  USP30 and parkin homeostatically regulate atypical ubiquitin chains on mitochondria.

Authors:  Christian N Cunningham; Joshua M Baughman; Lilian Phu; Joy S Tea; Christine Yu; Mary Coons; Donald S Kirkpatrick; Baris Bingol; Jacob E Corn
Journal:  Nat Cell Biol       Date:  2015-01-26       Impact factor: 28.824

Review 6.  Dissecting the mechanisms of cell division.

Authors:  Joseph Y Ong; Jorge Z Torres
Journal:  J Biol Chem       Date:  2019-06-07       Impact factor: 5.157

7.  The C7orf43/TRAPPC14 component links the TRAPPII complex to Rabin8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.

Authors:  Adrian Cuenca; Christine Insinna; Huijie Zhao; Peter John; Matthew A Weiss; Quanlong Lu; Vijay Walia; Suzanne Specht; Selvambigai Manivannan; Jimmy Stauffer; Andrew A Peden; Christopher J Westlake
Journal:  J Biol Chem       Date:  2019-08-29       Impact factor: 5.157

8.  Phosphorylation regulates the p31Comet-mitotic arrest-deficient 2 (Mad2) interaction to promote spindle assembly checkpoint (SAC) activity.

Authors:  Dipali A Date; Amy C Burrows; Matthew K Summers
Journal:  J Biol Chem       Date:  2014-03-04       Impact factor: 5.157

9.  Feasibility and biological rationale of repurposing sunitinib and erlotinib for dengue treatment.

Authors:  Szu-Yuan Pu; Fei Xiao; Stanford Schor; Elena Bekerman; Fabio Zanini; Rina Barouch-Bentov; Claude M Nagamine; Shirit Einav
Journal:  Antiviral Res       Date:  2018-05-16       Impact factor: 5.970

10.  Ubiquilin4 is an adaptor protein that recruits Ubiquilin1 to the autophagy machinery.

Authors:  Dong Yun Lee; David Arnott; Eric J Brown
Journal:  EMBO Rep       Date:  2013-03-05       Impact factor: 8.807
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