Literature DB >> 19404680

Expression of four pha genes involved in poly-beta-hydroxybutyrate production and accumulation in Rhodobacter sphaeroides FJ1.

Min-En Chou1, Wen-Tuan Chang, Ya-Chieh Chang, Mei-Kwei Yang.   

Abstract

A cluster of genes encoding polyhydroxybutyrate (PHB) depolymerase (phaZ), PHB synthase (phaC), phasin (phaP), and the regulator protein (phaR) was previously identified in Rhodobacter sphaeroides FJ1 (R. sphaeroides FJ1). In this study, we investigated the role of the PhaR protein on the expression of the pha genes. Immunoblot analysis revealed that the expressions of phaP, phaZ and phaR genes in wild-type cells of R. sphaeroides FJ1 are repressed during the active growth phase, with the exception of phaC. A phaR deletion mutant of R. sphaeroides FJ1 was constructed, and the basal level of phaP and phaZ expression in this mutant was markedly increased. Electrophoretic mobility shift assays demonstrated that PhaR binds to the promoter region of phaP as well as those of phaR and phaZ. These results suggest that the PhaR protein is a repressor of phaP, phaR, and phaZ genes in R. sphaeroides FJ1.

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Year:  2009        PMID： 19404680     DOI： 10.1007/s00438-009-0448-4

Source DB:  PubMed          Journal:  Mol Genet Genomics        ISSN： 1617-4623            Impact factor:   3.291


  27 in total

1.  Polyhydroxyalkanoate inclusion body-associated proteins and coding region in Bacillus megaterium.

Authors:  G J McCool; M C Cannon
Journal:  J Bacteriol       Date:  1999-01       Impact factor: 3.490

2.  New insight into the role of the PhaP phasin of Ralstonia eutropha in promoting synthesis of polyhydroxybutyrate.

Authors:  G M York; J Stubbe; A J Sinskey
Journal:  J Bacteriol       Date:  2001-04       Impact factor: 3.490

3.  Construction of a broad-host-range promoter-probing vector and cloning of promoter fragments of Xanthomonas campestris.

Authors:  S F Weng; M Y Shieh; F Y Lai; Y Y Shao; J W Lin; Y H Tseng
Journal:  Biochem Biophys Res Commun       Date:  1996-11-12       Impact factor: 3.575

4.  Analyses of a polyhydroxyalkanoic acid granule-associated 16-kilodalton protein and its putative regulator in the pha locus of Paracoccus denitrificans.

Authors:  A Maehara; S Ueda; H Nakano; T Yamane
Journal:  J Bacteriol       Date:  1999-05       Impact factor: 3.490

5.  The Ralstonia eutropha PhaR protein couples synthesis of the PhaP phasin to the presence of polyhydroxybutyrate in cells and promotes polyhydroxybutyrate production.

Authors:  Gregory M York; JoAnne Stubbe; Anthony J Sinskey
Journal:  J Bacteriol       Date:  2002-01       Impact factor: 3.490

Review 6.  Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic.

Authors:  L L Madison; G W Huisman
Journal:  Microbiol Mol Biol Rev       Date:  1999-03       Impact factor: 11.056

7.  5-Aminolevulinate production by Escherichia coli containing the Rhodobacter sphaeroides hemA gene.

Authors:  M J van der Werf; J G Zeikus
Journal:  Appl Environ Microbiol       Date:  1996-10       Impact factor: 4.792

8.  Identification of a 13-kDa protein associated with the polyhydroxyalkanoic acid granules from Acinetobacter spp.

Authors:  M A Schembri; A A Woods; R C Bayly; J K Davies
Journal:  FEMS Microbiol Lett       Date:  1995-11-15       Impact factor: 2.742

Review 9.  Molecular basis for biosynthesis and accumulation of polyhydroxyalkanoic acids in bacteria.

Authors:  A Steinbüchel; E Hustede; M Liebergesell; U Pieper; A Timm; H Valentin
Journal:  FEMS Microbiol Rev       Date:  1992-12       Impact factor: 16.408

10.  Purification and characterization of a 14-kilodalton protein that is bound to the surface of polyhydroxyalkanoic acid granules in Rhodococcus ruber.

Authors:  U Pieper-Fürst; M H Madkour; F Mayer; A Steinbüchel
Journal:  J Bacteriol       Date:  1994-07       Impact factor: 3.490
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  5 in total

1.  Construction of a Rhodobacter sphaeroides Strain That Efficiently Produces Hydrogen Gas from Acetate without Poly(β-Hydroxybutyrate) Accumulation: Insight into the Role of PhaR in Acetate Metabolism.

Authors:  Tetsu Shimizu; Haruhiko Teramoto; Masayuki Inui
Journal:  Appl Environ Microbiol       Date:  2022-06-07       Impact factor: 5.005

Review 2.  An updated overview on the regulatory circuits of polyhydroxyalkanoates synthesis.

Authors:  Ruchira Mitra; Tong Xu; Guo-Qiang Chen; Hua Xiang; Jing Han
Journal:  Microb Biotechnol       Date:  2021-09-02       Impact factor: 6.575

Review 3.  The Modification of Regulatory Circuits Involved in the Control of Polyhydroxyalkanoates Metabolism to Improve Their Production.

Authors:  Claudia Velázquez-Sánchez; Guadalupe Espín; Carlos Peña; Daniel Segura
Journal:  Front Bioeng Biotechnol       Date:  2020-04-30

4.  Proteomic responses of Roseobacter litoralis OCh149 to starvation and light regimen.

Authors:  Rui Zong; Nianzhi Jiao
Journal:  Microbes Environ       Date:  2012-10-05       Impact factor: 2.912

5.  Bradyrhizobium diazoefficiens USDA110 PhaR functions for pleiotropic regulation of cellular processes besides PHB accumulation.

Authors:  Shogo Nishihata; Takahiko Kondo; Kosei Tanaka; Shu Ishikawa; Shinji Takenaka; Choong-Min Kang; Ken-Ichi Yoshida
Journal:  BMC Microbiol       Date:  2018-10-24       Impact factor: 3.605
  5 in total

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