AIM: Neovascularization is an important event in proliferative diabetic retinopathy (PDR), where various secretory proteins including multiple growth factors are considered to be involved in this process. We searched for secretory proteins expressed in a surgical specimen obtained from the eyes of patients with PDR. METHODS: We developed the oligo-cap signal sequence trap (SST) strategy which enables us to screen for secretory or membrane proteins from a minimal starting material. Using this method, we were able to screen a cDNA library constructed from a surgical specimen obtained from the eyes of the patients with PDR. RESULTS: Majority of the cloned cDNAs turned out to encode secreted protein acidic and rich in cystein (SPARC), strongly suggesting that SPARC is highly expressed in PDR. Analysis of vitreous fluid from various patients has shown that the concentration of SPARC protein is increased in patients with PDR. Furthermore, subretinal injection of recombinant SPARC adenovirus induced PDR-like changes in the rat eye. CONCLUSIONS: Our results strongly suggested that SPARC is involved in the development of diabetic retinopathy (DR).
AIM: Neovascularization is an important event in proliferative diabetic retinopathy (PDR), where various secretory proteins including multiple growth factors are considered to be involved in this process. We searched for secretory proteins expressed in a surgical specimen obtained from the eyes of patients with PDR. METHODS: We developed the oligo-cap signal sequence trap (SST) strategy which enables us to screen for secretory or membrane proteins from a minimal starting material. Using this method, we were able to screen a cDNA library constructed from a surgical specimen obtained from the eyes of the patients with PDR. RESULTS: Majority of the cloned cDNAs turned out to encode secreted protein acidic and rich in cystein (SPARC), strongly suggesting that SPARC is highly expressed in PDR. Analysis of vitreous fluid from various patients has shown that the concentration of SPARC protein is increased in patients with PDR. Furthermore, subretinal injection of recombinant SPARC adenovirus induced PDR-like changes in the rat eye. CONCLUSIONS: Our results strongly suggested that SPARC is involved in the development of diabetic retinopathy (DR).
Authors: Li Jia Chen; Pancy O S Tam; Clement C Y Tham; Xiao Ying Liang; Sylvia W Y Chiang; Oscar Canlas; Robert Ritch; Douglas J Rhee; Chi Pui Pang Journal: Mol Vis Date: 2010-10-08 Impact factor: 2.367
Authors: Stefaniya Konstantinova Boneva; Julian Wolf; Rozina Ida Hajdú; Gabriele Prinz; Henrike Salié; Anja Schlecht; Saskia Killmer; Yannik Laich; Henrik Faatz; Albrecht Lommatzsch; Martin Busch; Felicitas Bucher; Andreas Stahl; Daniel Böhringer; Bertram Bengsch; Günther Schlunck; Hansjürgen Agostini; Clemens A K Lange Journal: Front Immunol Date: 2021-11-02 Impact factor: 7.561