| Literature DB >> 19402204 |
Peter Klucar1, Peter F Barnes, Ying Kong, Susan T Howard, Xiuhua Pang, Fang-Fang Huang, Amy R Tvinnereim, Buka Samten, Homayoun Shams.
Abstract
To determine the immunogenicity and protective efficacy of the Mycobacterium tuberculosis 10 kD culture filtrate protein (CFP10), and to evaluate strategies that enhance local immunity, we used C57Bl/6 DR4 mice that were transgenic for human HLA DRB1 0401, because CFP10 contains epitopes for DRB1 0401 but not for C57Bl/6 mice. Intramuscular immunization with a DNA vaccine encoding CFP10 elicited production of IFN-gamma by systemic CD4+ T cells, and one intravenous dose of the CFP10-based DNA vaccine coated with polyethylenimine (PEI) stimulated IFN-gamma production by lung CD4+ cells and reduced the pulmonary bacillary burden. We conclude that CFP10 is a potential vaccine candidate and that coating vaccines with PEI enhances local protective immunity to tuberculosisEntities:
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Year: 2009 PMID： 19402204 PMCID： PMC2768422 DOI： 10.1016/j.vaccine.2009.01.119
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641