BACKGROUND: Osteopontin (OPN) is an extracellular matrix protein with a wide range of functions, and is involved in various inflammatory diseases. However, the role of OPN in eosinophilic airway inflammation is unclear. OBJECTIVE: To elucidate the role of OPN in eosinophilic airway inflammation. METHODS: OPN protein levels in induced sputum from asthmatic patients and healthy controls were measured. Eosinophil migration assays were performed in the presence or absence of OPN, a blocking antibody (Ab) recognizing its integrin-binding domain (2K1) and an anti-integrin alpha 4 Ab (P1H4). In the mouse asthma model, the levels of eosinophilia were examined in bronchoalveolar lavage fluids (BALFs) from ovalbumin (OVA)-sensitized and -challenged mice with or without administration of an Ab (M5) corresponding to human 2K1. RESULTS: Levels of OPN in induced sputum were significantly higher in asthmatic patients when compared with healthy controls. In addition, levels of OPN were correlated with the percentage of sputum eosinophils. OPN induced significant migration of human eosinophils and this effect was inhibited by 2K1 and P1H4. M5 significantly attenuated OVA-induced eosinophilia in BALFs. CONCLUSION: These results indicate that OPN plays a role in the migration of eosinophils into the airways and may be involved in the pathogenesis of asthma.
BACKGROUND:Osteopontin (OPN) is an extracellular matrix protein with a wide range of functions, and is involved in various inflammatory diseases. However, the role of OPN in eosinophilic airway inflammation is unclear. OBJECTIVE: To elucidate the role of OPN in eosinophilic airway inflammation. METHODS:OPN protein levels in induced sputum from asthmatic patients and healthy controls were measured. Eosinophil migration assays were performed in the presence or absence of OPN, a blocking antibody (Ab) recognizing its integrin-binding domain (2K1) and an anti-integrin alpha 4 Ab (P1H4). In the mouseasthma model, the levels of eosinophilia were examined in bronchoalveolar lavage fluids (BALFs) from ovalbumin (OVA)-sensitized and -challenged mice with or without administration of an Ab (M5) corresponding to human 2K1. RESULTS: Levels of OPN in induced sputum were significantly higher in asthmatic patients when compared with healthy controls. In addition, levels of OPN were correlated with the percentage of sputum eosinophils. OPN induced significant migration of human eosinophils and this effect was inhibited by 2K1 and P1H4. M5 significantly attenuated OVA-induced eosinophilia in BALFs. CONCLUSION: These results indicate that OPN plays a role in the migration of eosinophils into the airways and may be involved in the pathogenesis of asthma.
Authors: Mehrdad Arjomandi; Josh M Galanter; Shweta Choudhry; Celeste Eng; Donglei Hu; Kenneth Beckman; Rocío Chapela; José R Rodríguez-Santana; William Rodríguez-Cintrón; Jean Ford; Pedro C Avila; Esteban G Burchard Journal: Pediatr Allergy Immunol Pulmonol Date: 2011-12 Impact factor: 1.349
Authors: Christopher J Wingard; Dianne M Walters; Brook L Cathey; Susana C Hilderbrand; Pranita Katwa; Sijie Lin; Pu Chun Ke; Ramakrishna Podila; Apparao Rao; Robert M Lust; Jared M Brown Journal: Nanotoxicology Date: 2010-11-03 Impact factor: 5.913
Authors: Brigitta Dombai; István Ivancsó; András Bikov; Dóra Oroszi; Anikó Bohács; Veronika Müller; János Rigó; Barna Vásárhelyi; György Losonczy; Lilla Tamási Journal: Can Respir J Date: 2017-10-23 Impact factor: 2.409