AIM: In the Amazon region of Brazil, the fruits of Caesalpinia ferrea Martius (Brazilian ironwood) are widely used as an antimicrobial and healing medicine in many situations including oral infections. This study aimed to evaluate the antimicrobial activity of Caesalpinia ferrea Martius fruit extract against oral pathogens. MATERIALS AND METHODS: Polyphenols estimation and spectral analysis ((1)H NMR) of the methanol extract were carried out. The microorganisms Candida albicans, Streptococcus mutans, Streptococcus salivarius, Streptococcus oralis and Lactobacillus casei were tested using the microdilution method for planktonic cells (MIC) and a multispecies biofilm model. Chlorhexidine was used as positive control. RESULTS: Polyphenols in the extract were estimated at 7.3% and (1)H NMR analysis revealed hydroxy phenols and methoxilated compounds. MIC values for Candida albicans, Streptococcus mutans, Streptococcus salivarius, Streptococcus oralis and Lactobacillus casei were 25.0, 40.0, 66.0, 100.0, 66.0 microg/mL, respectively. For the biofilm assay, chlorhexidine and plant extract showed no growth at 10(-4) and 10(-5) microbial dilution, respectively. At 10(-4) and 10(-5) the growth values (mean+/-SD) of the negative controls (DMSO and saline solution) for Streptococcus mutans, Streptococcus sp. and Candida albicans were 8.1+/-0.7, 7.0+/-0.6 and 5.9+/-0.9 x 10(6)CFU, respectively. CONCLUSION: Caesalpinia ferrea fruit extract can inhibit in vitro growth of oral pathogens in planktonic and biofilm models supporting its use for oral infections.
AIM: In the Amazon region of Brazil, the fruits of Caesalpinia ferrea Martius (Brazilian ironwood) are widely used as an antimicrobial and healing medicine in many situations including oral infections. This study aimed to evaluate the antimicrobial activity of Caesalpinia ferrea Martius fruit extract against oral pathogens. MATERIALS AND METHODS:Polyphenols estimation and spectral analysis ((1)H NMR) of the methanol extract were carried out. The microorganisms Candida albicans, Streptococcus mutans, Streptococcus salivarius, Streptococcus oralis and Lactobacillus casei were tested using the microdilution method for planktonic cells (MIC) and a multispecies biofilm model. Chlorhexidine was used as positive control. RESULTS:Polyphenols in the extract were estimated at 7.3% and (1)H NMR analysis revealed hydroxy phenols and methoxilated compounds. MIC values for Candida albicans, Streptococcus mutans, Streptococcus salivarius, Streptococcus oralis and Lactobacillus casei were 25.0, 40.0, 66.0, 100.0, 66.0 microg/mL, respectively. For the biofilm assay, chlorhexidine and plant extract showed no growth at 10(-4) and 10(-5) microbial dilution, respectively. At 10(-4) and 10(-5) the growth values (mean+/-SD) of the negative controls (DMSO and saline solution) for Streptococcus mutans, Streptococcus sp. and Candida albicans were 8.1+/-0.7, 7.0+/-0.6 and 5.9+/-0.9 x 10(6)CFU, respectively. CONCLUSION:Caesalpinia ferrea fruit extract can inhibit in vitro growth of oral pathogens in planktonic and biofilm models supporting its use for oral infections.
Authors: Ana Carolina A V Kayano; Stefanie C P Lopes; Fernanda G Bueno; Elaine C Cabral; Wanessa C Souza-Neiras; Lucy M Yamauchi; Mary A Foglio; Marcos N Eberlin; João Carlos P Mello; Fabio T M Costa Journal: Malar J Date: 2011-05-02 Impact factor: 2.979
Authors: Raquel de Oliveira Marreiro; Maria Fulgência Costa Lima Bandeira; Tatiane Pereira de Souza; Mailza Costa de Almeida; Katiana Bendaham; Gisely Naura Venâncio; Isis Costa Rodrigues; Cristiane Nagai Coelho; Patrícia Sâmea Lêdo Lima Milério; Glauber Palma de Oliveira; Nikeila Chacon de Oliveira Conde Journal: Clin Cosmet Investig Dent Date: 2014-01-28
Authors: Magda R A Ferreira; Mônica T M Fernandes; Wliana A V da Silva; Isabelle C F Bezerra; Tatiane P de Souza; Maria F Pimentel; Luiz A L Soares Journal: Pharmacogn Mag Date: 2016-05-11 Impact factor: 1.085
Authors: Márcia Maria Mendes Marques; Selene Maia de Morais; Ana Raquel Araújo da Silva; Naiara Dutra Barroso; Tadeu Rocha Pontes Filho; Fernanda Montenegro de Carvalho Araújo; Ícaro Gusmão Pinto Vieira; Danielle Malta Lima; Maria Izabel Florindo Guedes Journal: Evid Based Complement Alternat Med Date: 2015-07-14 Impact factor: 2.629