| Literature DB >> 19393698 |
Anees A Ansari1, Pratima R Solanki, B D Malhotra.
Abstract
Nanostructured cerium oxide (NanoCeO(2)) film deposited onto indium-tin-oxide (ITO) glass substrate by solution casting has been used for immobilization of horseradish peroxidase (HRP) via physiosorption technique. X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-vis and electrochemical techniques have been utilized for characterization of NanoCeO(2)/ITO electrode and HRP/NanoCeO(2)/ITO bioelectrode. The HRP/NanoCeO(2)/ITO electrode exhibits value of the apparent Michaelis-Menten constant (K(m)) as 2.21 microM, linear regression coefficient as 0.998 and linearity for hydrogen peroxide as 1.0-170 microM obtained using electrochemical response measurements. Besides this, HRP/NanoCeO(2)/ITO bioelectrode can be used about 20 times and is stable for 5 weeks at 4 degrees C. The results of photo-response studies carried out on HRP/NanoCeO(2)/ITO bioelectrode indicate reasonable agreement with those obtained using amperometric technique.Entities:
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Year: 2009 PMID： 19393698 DOI： 10.1016/j.jbiotec.2009.04.005
Source DB: PubMed Journal: J Biotechnol ISSN： 0168-1656 Impact factor: 3.307