| Literature DB >> 19393590 |
Steve K W Oh1, Allen K Chen, Yanglin Mok, Xiaoli Chen, U-Ming Lim, Angela Chin, Andre B H Choo, Shaul Reuveny.
Abstract
The conventional method of culturing human embryonic stem cells (hESC) is on two-dimensional (2D) surfaces, which is not amenable for scale up to therapeutic quantities in bioreactors. We have developed a facile and robust method for maintaining undifferentiated hESC in three-dimensional (3D) suspension cultures on matrigel-coated microcarriers achieving 2- to 4-fold higher cell densities than those in 2D colony cultures. Stable, continuous propagation of two hESC lines on microcarriers has been demonstrated in conditioned media for 6 months. Microcarrier cultures (MC) were also demonstrated in two serum-free defined media (StemPro and mTeSR1). MC achieved even higher cell concentrations in suspension spinner flasks, thus opening the prospect of propagation in controlled bioreactors.Entities:
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Year: 2009 PMID： 19393590 DOI： 10.1016/j.scr.2009.02.005
Source DB: PubMed Journal: Stem Cell Res ISSN： 1873-5061 Impact factor: 2.020