Literature DB >> 19390736

Immunoperoxidase technique using an anti-Leishmania (L.) chagasi hyperimmune serum in the diagnosis of culture-confirmed American tegumentary leishmaniasis.

Leonardo P Quintella1, Tullia Cuzzi, Maria de F Madeira, Thais Okamoto, Armando de O Schubach.   

Abstract

The present study reports the production of the rabbit anti-Leishmania (L.) chagasi hyperimmune serum, the standardization of the immunohistochemistry (IHC) technique and the evaluation of its employment in cutaneous leishmaniasis (CL) lesions diagnosed by Leishmania sp. culture isolation. Thirty fragments of active CL lesions were examined as well as 10 fragments of cutaneous mycosis lesions as control group. IHC proved more sensitive in detecting amastigotes than conventional hematoxylin-eosin (HE) stained slides: the former was positive in 24 (80%) biopsies whereas the latter, in 16 (53%) (p = 0.028). The reaction stained different fungus species causing cutaneous mycosis. Besides, positive reaction was noticed in mononuclear and endothelial cells. Nevertheless, this finding was present in the control group biopsies. It is concluded that IHC showed good sensitivity in detecting amastigotes.

Entities:  

Mesh：

Substances：

Year:  2009        PMID： 19390736     DOI： 10.1590/s0036-46652009000200005

Source DB:  PubMed          Journal:  Rev Inst Med Trop Sao Paulo        ISSN： 0036-4665            Impact factor:   1.846


  10 in total

1.  IDENTIFICATION OF CANINE VISCERAL LEISHMANIASIS IN A PREVIOUSLY UNAFFECTED AREA BY CONVENTIONAL DIAGNOSTIC TECHNIQUES AND CELL-BLOCK FIXATION.

Authors:  Tuanne Rotti Abrantes; Maria de Fátima Madeira; Denise Amaro da Silva; Carolina dos Santos F S Perié; Artur Augusto V Mendes Júnior; Rodrigo Caldas Menezes; Valmir Laurentino Silva; Fabiano Borges Figueiredo
Journal:  Rev Inst Med Trop Sao Paulo       Date:  2016-02-23       Impact factor: 1.846

2.  Imaging Leishmania major Antigens in Experimentally Infected Macrophages and Dermal Scrapings from Cutaneous Leishmaniasis Lesions in Tunisia.

Authors:  Nasreddine Saïdi; Yousr Galaï; Meriem Ben-Abid; Thouraya Boussoffara; Ines Ben-Sghaier; Karim Aoun; Aïda Bouratbine
Journal:  Microorganisms       Date:  2022-06-04

3.  Sensitivity and specificity of in situ hybridization for diagnosis of cutaneous infection by Leishmania infantum in dogs.

Authors:  Rodrigo C Menezes; Fabiano B Figueiredo; Annabel G Wise; Maria F Madeira; Raquel V C Oliveira; Tânia M P Schubach; Matti Kiupel; Ingeborg M Langohr
Journal:  J Clin Microbiol       Date:  2012-11-07       Impact factor: 5.948

4.  Are Neutrophil Extracellular Traps Playing a Role in the Parasite Control in Active American Tegumentary Leishmaniasis Lesions?

Authors:  Fernanda Nazaré Morgado; Michelle T C Nascimento; Elvira M Saraiva; Carla de Oliveira-Ribeiro; Maria de Fátima Madeira; Marcela da Costa-Santos; Erica C F Vasconcellos; Maria Ines F Pimentel; Marcelo Rosandiski Lyra; Armando de Oliveira Schubach; Fátima Conceição-Silva
Journal:  PLoS One       Date:  2015-07-20       Impact factor: 3.240

5.  Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection.

Authors:  Mauro Maciel de Arruda; Fabiano Borges Figueiredo; Andreza Pain Marcelino; José Ronaldo Barbosa; Guilherme Loureiro Werneck; Elza Ferreira Noronha; Gustavo Adolfo Sierra Romero
Journal:  Mem Inst Oswaldo Cruz       Date:  2016-02-19       Impact factor: 2.743

6.  Comparative study of in situ hybridization, immunohistochemistry and parasitological culture for the diagnosis of canine leishmaniosis.

Authors:  Marina C Furtado; Rodrigo C Menezes; Matti Kiupel; Maria F Madeira; Raquel V C Oliveira; Ingeborg M Langohr; Fabiano B Figueiredo
Journal:  Parasit Vectors       Date:  2015-12-02       Impact factor: 3.876

7.  Sporotrichoid leishmaniasis: a cross-sectional clinical, epidemiological and laboratory study in Rio de Janeiro State, Brazil.

Authors:  Livia Martins Veloso de Carvalho; Maria Inês Fernandes Pimentel; Fátima Conceição-Silva; Érica de Camargo Ferreira E Vasconcellos; Cláudia Maria Valete-Rosalino; Marcelo Rosandiski Lyra; Mariza de Matos Salgueiro; Maurício Naoto Saheki; Maria de Fátima Madeira; Eliame Mouta-Confort; Liliane de Fátima Antonio; Aline Fagundes da Silva; Leonardo Pereira Quintella; Sandro Javier Bedoya-Pacheco; Armando de Oliveira Schubach
Journal:  Rev Inst Med Trop Sao Paulo       Date:  2017-06-01       Impact factor: 1.846

8.  Anti-mitochondrial Tryparedoxin Peroxidase Monoclonal Antibody-Based Immunohistochemistry for Diagnosis of Cutaneous Leishmaniasis.

Authors:  Mariana Lourenço Freire; Felipe Dutra Rego; Karine Ferreira Lopes; Lucélia Antunes Coutinho; Rafaella Fortini Queiroz Grenfell; Daniel Moreira Avelar; Gláucia Cota; Marcelo Antônio Pascoal-Xavier; Edward Oliveira
Journal:  Front Microbiol       Date:  2022-02-28       Impact factor: 5.640

9.  American tegumentary leishmaniasis: effectiveness of an immunohistochemical protocol for the detection of Leishmania in skin.

Authors:  Cibele Fontes Alves; Cintia Fontes Alves; Maria Marta Figueiredo; Carolina Carvalho Souza; George Luiz Lins Machado-Coelho; Maria Norma Melo; Washington Luiz Tafuri; Pedro Raso; Rodrigo Pedro Soares; Wagner Luiz Tafuri
Journal:  PLoS One       Date:  2013-05-21       Impact factor: 3.240

10.  Histopathological characteristics of cutaneous lesions caused by Leishmania Viannia panamensis in Panama.

Authors:  Kadir González; Rosendo Diaz; Aurea F Ferreira; Víctor García; Héctor Paz; José E Calzada; Michelle Ruíz; Márcia Laurenti; Azael Saldaña
Journal:  Rev Inst Med Trop Sao Paulo       Date:  2018-02-15       Impact factor: 1.846
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.