Literature DB >> 1938966

Positive selection for uracil auxotrophs of the sulfur-dependent thermophilic archaebacterium Sulfolobus acidocaldarius by use of 5-fluoroorotic acid.

S Kondo1, A Yamagishi, T Oshima.   

Abstract

Uracil auxotrophs of Sulfolobus acidocaldarius were positively selected by using 5-fluoroorotic acid. The wild-type strain was unable to grow in medium containing 5-fluoroorotic acid, whereas the mutants grew normally. Positive selection could be done for the auxotrophs. Mutants deficient in orotidine-5'-monophosphate pyrophosphorylase activity were isolated.

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Year:  1991        PMID： 1938966      PMCID： PMC212540          DOI： 10.1128/jb.173.23.7698-7700.1991

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  15 in total

1.  Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli.

Authors:  J R BECKWITH; A B PARDEE; R AUSTRIAN; F JACOB
Journal:  J Mol Biol       Date:  1962-12       Impact factor: 5.469

2.  High Efficiency of Plating of the Thermophilic Sulfur-Dependent Archaebacterium Sulfolobus acidocaldarius.

Authors:  E B Lindström; H M Sehlin
Journal:  Appl Environ Microbiol       Date:  1989-11       Impact factor: 4.792

3.  Positive screening and transformation of ura5 mutants in the fungus Podospora anserina: characterization of the transformants.

Authors:  V Razanamparany; J Bégueret
Journal:  Curr Genet       Date:  1986       Impact factor: 3.886

4.  Selection and characterization of ura5 mutants of Histoplasma capsulatum.

Authors:  P L Worsham; W E Goldman
Journal:  Mol Gen Genet       Date:  1988-10

5.  Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature.

Authors:  T D Brock; K M Brock; R T Belly; R L Weiss
Journal:  Arch Mikrobiol       Date:  1972

6.  Genetic transfer in Halobacterium volcanii.

Authors:  M Mevarech; R Werczberger
Journal:  J Bacteriol       Date:  1985-04       Impact factor: 3.490

7.  Transformation of Halobacterium halobium: development of vectors and investigation of gas vesicle synthesis.

Authors:  U Blaseio; F Pfeifer
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

8.  Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker.

Authors:  C Grimm; J Kohli; J Murray; K Maundrell
Journal:  Mol Gen Genet       Date:  1988-12

9.  Characterization of the small endogenous plasmid of Halobacterium strain SB3 and its use in transformation of H. halobium.

Authors:  N R Hackett; S DasSarma
Journal:  Can J Microbiol       Date:  1989-01       Impact factor: 2.419

10.  Transformation of the archaebacterium Halobacterium volcanii with genomic DNA.

Authors:  S W Cline; L C Schalkwyk; W F Doolittle
Journal:  J Bacteriol       Date:  1989-09       Impact factor: 3.490
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  18 in total

1.  High spontaneous mutation rate in the hyperthermophilic archaeon Sulfolobus solfataricus is mediated by transposable elements.

Authors:  E Martusewitsch; C W Sensen; C Schleper
Journal:  J Bacteriol       Date:  2000-05       Impact factor: 3.490

2.  Mutations and rearrangements in the genome of Sulfolobus solfataricus P2.

Authors:  Peter Redder; Roger A Garrett
Journal:  J Bacteriol       Date:  2006-06       Impact factor: 3.490

3.  Targeted gene disruption by homologous recombination in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1.

Authors:  Takaaki Sato; Toshiaki Fukui; Haruyuki Atomi; Tadayuki Imanaka
Journal:  J Bacteriol       Date:  2003-01       Impact factor: 3.490

4.  Construction of a shuttle vector for, and spheroplast transformation of, the hyperthermophilic archaeon Pyrococcus abyssi.

Authors:  Soizick Lucas; Laurent Toffin; Yvan Zivanovic; Daniel Charlier; Hélène Moussard; Patrick Forterre; Daniel Prieur; Gaël Erauso
Journal:  Appl Environ Microbiol       Date:  2002-11       Impact factor: 4.792

5.  Molecular biology of extremophiles.

Authors:  M Ciaramella; R Cannio; M Moracci; F M Pisani; M Rossi
Journal:  World J Microbiol Biotechnol       Date:  1995-01       Impact factor: 3.312

6.  Identification of the UMP synthase gene by establishment of uracil auxotrophic mutants and the phenotypic complementation system in the marine diatom Phaeodactylum tricornutum.

Authors:  Toshiro Sakaguchi; Kensuke Nakajima; Yusuke Matsuda
Journal:  Plant Physiol       Date:  2011-03-02       Impact factor: 8.340

7.  Rates of spontaneous mutation in an archaeon from geothermal environments.

Authors:  K L Jacobs; D W Grogan
Journal:  J Bacteriol       Date:  1997-05       Impact factor: 3.490

8.  An insertion element of the extremely thermophilic archaeon Sulfolobus solfataricus transposes into the endogenous beta-galactosidase gene.

Authors:  C Schleper; R Röder; T Singer; W Zillig
Journal:  Mol Gen Genet       Date:  1994-04

9.  Metal resistance and lithoautotrophy in the extreme thermoacidophile Metallosphaera sedula.

Authors:  Yukari Maezato; Tyler Johnson; Samuel McCarthy; Karl Dana; Paul Blum
Journal:  J Bacteriol       Date:  2012-10-12       Impact factor: 3.490

10.  Mutation and reversion frequencies of different Sulfolobus species and strains.

Authors:  Silvia Berkner; Georg Lipps
Journal:  Extremophiles       Date:  2008-01-05       Impact factor: 2.395
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