| Literature DB >> 1938675 |
N Raja1, M Goodson, D G Smith, R J Rowbury.
Abstract
A study of the conjugal transfer of ColV,I-K94 tn 10 from acid-treated donors suggested that acid-habituated recipients repair acid-damaged plasmid DNA better than those that are not habituated. The presence of an increased repair activity for acid-damaged DNA in habituated cells was confirmed by isolating pBR322 from acid-treated organisms; habituated cells produced more transformants when transformed by it than did non-habituated ones. Additionally, agarose gel electrophoretic studies of pBR322 DNA isolated from acid-damaged cells and tests of its transforming activity both indicated that plasmid DNA in habituated cells is less damaged by extreme acidity than is that in non-habituated organisms.Entities:
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Year: 1991 PMID： 1938675 DOI： 10.1111/j.1365-2672.1991.tb02748.x
Source DB: PubMed Journal: J Appl Bacteriol ISSN： 0021-8847