| Literature DB >> 19386715 |
Pim L J van der Heiden1, Renate de Boer, Dirk M van der Steen, Michel G D Kester, Menno W A G van der Hoorn, Wilmy M E Haarman, Helen E Barnby-Porritt, Jeremy W Fry, C E Napper, Erik W A Marijt, Roel Willemze, J H Frederik Falkenburg, Mirjam H M Heemskerk.
Abstract
To study the role of CD8 T cells in the control of varicella-zoster virus (VZV) reactivation, we developed multimeric major histocompatibility complexes to identify VZV-specific CD8 T cells. Potential HLA-A2 binding peptides from the putative immediate-early 62 protein (IE62) of VZV were tested for binding, and peptides with sufficient binding capacity were used to generate pentamers. Patients with VZV reactivation following stem cell transplantation were screened with these pentamers, leading to the identification of the first validated class I-restricted epitope of VZV. In 42% of HLA-A2 patients following VZV reactivation, these IE62-ALW-A2 T cells could be detected ex vivo.Entities:
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Year: 2009 PMID： 19386715 PMCID： PMC2704790 DOI： 10.1128/JVI.02662-08
Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103