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Literature DB >> 1938485

Cytometric analysis of DNA replication inhibited by emetine and cyclosporin A.

T Schweighoffer¹, E Schweighoffer, A Apati, F Antoni, G Molnar, K Lapis, G Banfalvi.

Abstract

DNA staining methods based on aspecific interactions with dye molecules have been replaced by an immunofluorescent approach to measure DNA replication. Biotin-11-dUTP was incorporated into permeable thymocytes isolated after emetine or cyclosporin A treatment of mice. Active sites of DNA replication were amplified based on biotin-avidin interaction and verified under fluorescent microscope. Cytometry of fluorescent images allow the direct measurement of replicating DNA without aspecific detection of total cellular DNA. Cytometric analysis of replication revealed that emetine acts at the early S phase, while cyclosporin A blocks in vivo DNA synthesis at mid S phase.

Entities: Chemical Species

Mesh：

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Year: 1991 PMID： 1938485 DOI： 10.1007/bf00266767

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

28 in total

1. Early replication signals in nuclei of Chinese hamster ovary cells.

Authors: G Banfalvi; H Tanke; A K Raap; J Slats; M van der Ploeg
Journal: Histochemistry Date: 1990

2. Structural basis for inhibition of protein synthesis by emetine and cycloheximide based on an analogy between ipecac alkaloids and glutarimide antibiotics.

Authors: A P Grollman
Journal: Proc Natl Acad Sci U S A Date: 1966-12 Impact factor: 11.205

3. Mapping replication units in animal cells.

Authors: S Handeli; A Klar; M Meuth; H Cedar
Journal: Cell Date: 1989-06-16 Impact factor: 41.582

4. Contribution of DNA polymerase delta to DNA replication in permeable CHO cells synchronized in S phase.

Authors: A Basnakian; G Banfalvi; N Sarkar
Journal: Nucleic Acids Res Date: 1989-06-26 Impact factor: 16.971

5. Structural changes in the mouse thymus and lymph node after emetine treatment.

Authors: I Oláh; F Antoni; G Bánfalvi; I Csuka; N G Luat; I Töró
Journal: Dev Comp Immunol Date: 1988 Impact factor: 3.636

6. Inhibitors of protein biosynthesis. V. Effects of emetine on protein and nucleic acid biosynthesis in HeLa cells.

Authors: A P Grollman
Journal: J Biol Chem Date: 1968-08-10 Impact factor: 5.157

7. Cellular regulation of ADP-ribosylation of proteins. III. Selective augmentation of in vitro ADP-ribosylation of histone H3 in murine thymic cells after in vivo emetine treatment.

Authors: A Sóoki-Tóth; G Bánfalvi; J Szöllösi; E Kirsten; M Staub; F Antoni; E Kun
Journal: Exp Cell Res Date: 1989-09 Impact factor: 3.905

Cytometric analysis of DNA replication inhibited by emetine and cyclosporin A.

1. Early replication signals in nuclei of Chinese hamster ovary cells.

2. Structural basis for inhibition of protein synthesis by emetine and cycloheximide based on an analogy between ipecac alkaloids and glutarimide antibiotics.

3. Mapping replication units in animal cells.

4. Contribution of DNA polymerase delta to DNA replication in permeable CHO cells synchronized in S phase.

5. Structural changes in the mouse thymus and lymph node after emetine treatment.

6. Inhibitors of protein biosynthesis. V. Effects of emetine on protein and nucleic acid biosynthesis in HeLa cells.

7. Cellular regulation of ADP-ribosylation of proteins. III. Selective augmentation of in vitro ADP-ribosylation of histone H3 in murine thymic cells after in vivo emetine treatment.

8. The immunosuppressive effect of acute doses of emetine on murine thymic cells.

9. Abnormal differentiation of thymocytes in mice treated with cyclosporin A.

10. Nuclei act as independent and integrated units of replication in a Xenopus cell-free DNA replication system.

Review 1. Biologically active isoquinoline alkaloids covering 2014-2018.

2. Effects of cyclosporin A on rat osteoblasts (ROS 17/2.8 cells) in vitro.

3. Emetine induces chemosensitivity and reduces clonogenicity of acute myeloid leukemia cells.