Literature DB >> 19381952

High-throughput analysis of serum antigens using sandwich ELISAs on microarrays.

Shannon L Servoss1, Rachel Gonzalez, Susan Varnum, Richard C Zangar.   

Abstract

Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection and validation of disease biomarkers. ELISA microarrays are capable of simultaneous detection of many proteins using a small sample volume. Although there are many potential pitfalls to the use of ELISA microarrays, these can be avoided by careful planning of experiments. In this chapter we describe a high-throughput protocol for processing ELISA microarrays that will result in reliable and reproducible data.

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Year:  2009        PMID： 19381952     DOI： 10.1007/978-1-60327-811-9_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  Dynamic secretome of bone marrow-derived stromal cells reveals a cardioprotective biochemical cocktail.

Authors:  David D Ellison; Yasir Suhail; Junaid Afzal; Laura Woo; Onur Kilic; Jeffrey Spees; Andre Levchenko
Journal:  Proc Natl Acad Sci U S A       Date:  2019-06-25       Impact factor: 11.205

2.  Susceptibility to quantum dot induced lung inflammation differs widely among the Collaborative Cross founder mouse strains.

Authors:  David K Scoville; Collin C White; Dianne Botta; Lisa A McConnachie; Megan E Zadworny; Stefanie C Schmuck; Xiaoge Hu; Xiaohu Gao; Jianbo Yu; Russell L Dills; Lianne Sheppard; Martha A Delaney; William C Griffith; Richard P Beyer; Richard C Zangar; Joel G Pounds; Elaine M Faustman; Terrance J Kavanagh
Journal:  Toxicol Appl Pharmacol       Date:  2015-10-21       Impact factor: 4.219

Review 3.  Photonic crystal enhanced fluorescence for early breast cancer biomarker detection.

Authors:  Brian T Cunningham; Richard C Zangar
Journal:  J Biophotonics       Date:  2012-06-27       Impact factor: 3.207

4.  Reactive oxygen species alter autocrine and paracrine signaling.

Authors:  Richard C Zangar; Nikki Bollinger; Thomas J Weber; Ruimin M Tan; L Meng Markillie; Norman J Karin
Journal:  Free Radic Biol Med       Date:  2011-09-10       Impact factor: 7.376

5.  Use of a single-chain antibody library for ovarian cancer biomarker discovery.

Authors:  Arturo B Ramirez; Christian M Loch; Yuzheng Zhang; Yan Liu; Xiaohong Wang; Elizabeth A Wayner; Jonathon E Sargent; Sahar Sibani; Eugenie Hainsworth; Eliseo A Mendoza; Ralph Eugene; Joshua Labaer; Nicole D Urban; Martin W McIntosh; Paul D Lampe
Journal:  Mol Cell Proteomics       Date:  2010-05-13       Impact factor: 5.911

6.  Reduction of Acute Inflammatory Effects of Fumed Silica Nanoparticles in the Lung by Adjusting Silanol Display through Calcination and Metal Doping.

Authors:  Bingbing Sun; Suman Pokhrel; Darren R Dunphy; Haiyuan Zhang; Zhaoxia Ji; Xiang Wang; Meiying Wang; Yu-Pei Liao; Chong Hyun Chang; Juyao Dong; Ruibin Li; Lutz Mädler; C Jeffrey Brinker; André E Nel; Tian Xia
Journal:  ACS Nano       Date:  2015-07-29       Impact factor: 15.881

7.  Coupled external cavity photonic crystal enhanced fluorescence.

Authors:  Anusha Pokhriyal; Meng Lu; Chun Ge; Brian T Cunningham
Journal:  J Biophotonics       Date:  2012-11-06       Impact factor: 3.207

8.  Spatially selective photonic crystal enhanced fluorescence and application to background reduction for biomolecule detection assays.

Authors:  Vikram Chaudhery; Cheng-Sheng Huang; Anusha Pokhriyal; James Polans; Brian T Cunningham
Journal:  Opt Express       Date:  2011-11-07       Impact factor: 3.894

Review 9.  Protein microarrays for personalized medicine.

Authors:  Xiaobo Yu; Nicole Schneiderhan-Marra; Thomas O Joos
Journal:  Clin Chem       Date:  2010-01-14       Impact factor: 8.327
  9 in total

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