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Literature DB >> 19381478

Staphylococcus cohnii hemolysins - isolation, purification and properties.

Abstract

A total 355 of Staphylococcus cohnii isolates from hospital environment, patients (newborns), medical staff and from non-hospital environment were tested for hemolytic activity. Ninety-one % of S. cohnii ssp. cohnii and 74.5 % S. cohnii ssp. urealyticus strains exhibited hemolysis synergistic to S. aureus ATCC 25923 strain. Crude preparations of hemolysins of both bacterial subspecies presented delta-hemolysin, but not alpha- and beta-toxin activity. Highly pure hemolysins were obtained by semipreparative SDS-PAGE or by organic solvent extraction from the freeze-dried crude preparations. Native-PAGE and 2D-PAGE showed their high heterogeneity. Molar masses of single hemolysin units estimated by the Tris-Tricine-SDS-PAGE were calculated as 3.47 kDa for S. cohnii ssp. cohnii and 3.53 kDa for S. cohnii ssp. urealyticus.

Entities: Chemical Disease Species

Mesh：

Substances：
Hemolysin Proteins

Year: 2009 PMID： 19381478 DOI： 10.1007/s12223-008-0082-2

Source DB: PubMed Journal: Folia Microbiol (Praha) ISSN： 0015-5632 Impact factor: 2.099

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Staphylococcus cohnii hemolysins - isolation, purification and properties.

Review 1. Exotoxins of Staphylococcus aureus.

2. Staphylococcus aureus biofilm as a target for single or repeated doses of oxacillin, vancomycin, linezolid and/or lysostaphin.

3. Staphylococcus cohnii septicaemia in a patient with colon cancer.

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5. A new method for the preparation and some properties of staphylococcal delta-haemolysin.

6. Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis.

7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

8. Coagulase-negative staphylococcal sepsis in neonates. Association between antibiotic resistance, biofilm formation and the host inflammatory response.

9. Synergistic hemolysis exhibited by species of staphylococci.

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