| Literature DB >> 19381396 |
Anees A Ansari1, Renu Singh, G Sumana, B D Malhotra.
Abstract
A 20-mer thiolated oligonucleotide probe (th-ssDNA) specific to Neisseria gonorrhoeae immobilized onto a sol-gel derived nano-structured zinc oxide (ZnO) film dip-coated onto an indium-tin-oxide (ITO) glass substrate has been used for the fabrication of a DNA biosensor for sexually transmitted disease (gonorrhoea) detection using hybridization technique. The results of characterization studies carried out on this th-ssDNA-ZnO/ITO bioelectrode using X-ray diffraction, UV-Visible, Fourier-transform infrared, scanning electron microscopy and electrochemical techniques reveal the linearity as 0.000524 fmol-0.524 nmol, with a detection limit of 0.000704 fmol within 60 s.Entities:
Mesh：
Substances：
Year: 2009 PMID： 19381396 DOI： 10.1039/b817562d
Source DB: PubMed Journal: Analyst ISSN： 0003-2654 Impact factor: 4.616