Literature DB >> 1937821

Sexual stage development of cryptosporidia in the Caco-2 cell line.

M Buraud1, E Forget, L Favennec, J Bizet, J G Gobert, A M Deluol.   

Abstract

We used the spontaneously differentiated human intestinal epithelial cell line Caco-2 to develop an in vitro model of Cryptosporidium sp. infection. The mean cell infection rate was 3% +/- 2%. Asexual stages of cryptosporidia were observed on day 2 postinoculation. Transmission electron microscopy showed the presence of macrogametes at day 5. This cell line appears to be suited to the study of the mechanisms by which biological agents inhibit both sexual and asexual development of cryptosporidia.

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Year:  1991        PMID： 1937821      PMCID： PMC259085          DOI： 10.1128/iai.59.12.4610-4613.1991

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  6 in total

1.  In vitro cultivation of Cryptosporidium parvum and screening for anticryptosporidial drugs.

Authors:  V McDonald; R Stables; D C Warhurst; M R Barer; D A Blewett; H D Chapman; G M Connolly; P L Chiodini; K P McAdam
Journal:  Antimicrob Agents Chemother       Date:  1990-08       Impact factor: 5.191

2.  Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients.

Authors:  M J Arrowood; C R Sterling
Journal:  J Parasitol       Date:  1987-04       Impact factor: 1.276

3.  Complete development of Cryptosporidium in cell culture.

Authors:  W L Current; T B Haynes
Journal:  Science       Date:  1984-05-11       Impact factor: 47.728

4.  Development of human and calf Cryptosporidium in chicken embryos.

Authors:  W L Current; P L Long
Journal:  J Infect Dis       Date:  1983-12       Impact factor: 5.226

5.  Asexual development of Cryptosporidium parvum within a differentiated human enterocyte cell line.

Authors:  T P Flanigan; T Aji; R Marshall; R Soave; M Aikawa; C Kaetzel
Journal:  Infect Immun       Date:  1991-01       Impact factor: 3.441

Review 6.  Cryptosporidiosis in perspective.

Authors:  S Tzipori
Journal:  Adv Parasitol       Date:  1988       Impact factor: 3.870
  6 in total
  12 in total

1.  Expression of receptors for enterotoxigenic Escherichia coli during enterocytic differentiation of human polarized intestinal epithelial cells in culture.

Authors:  S Kernéis; G Chauvière; A Darfeuille-Michaud; D Aubel; M H Coconnier; B Joly; A L Servin
Journal:  Infect Immun       Date:  1992-07       Impact factor: 3.441

2.  In vitro infection of Cryptosporidium parvum to four different cell lines.

Authors:  J R Yu; S D Choi; Y W Kim
Journal:  Korean J Parasitol       Date:  2000-06       Impact factor: 1.341

3.  Cryptosporidium parvum infection of human intestinal epithelial cells induces the polarized secretion of C-X-C chemokines.

Authors:  F Laurent; L Eckmann; T C Savidge; G Morgan; C Theodos; M Naciri; M F Kagnoff
Journal:  Infect Immun       Date:  1997-12       Impact factor: 3.441

4.  Characterization of an intestinal epithelial cell receptor recognized by the Cryptosporidium parvum sporozoite ligand CSL.

Authors:  R C Langer; D A Schaefer; M W Riggs
Journal:  Infect Immun       Date:  2001-03       Impact factor: 3.441

5.  Attachment of Cryptosporidium parvum sporozoites to MDCK cells in vitro.

Authors:  D H Hamer; H Ward; S Tzipori; M E Pereira; J P Alroy; G T Keusch
Journal:  Infect Immun       Date:  1994-06       Impact factor: 3.441

6.  Effects of select medium supplements on in vitro development of Cryptosporidium parvum in HCT-8 cells.

Authors:  S J Upton; M Tilley; D B Brillhart
Journal:  J Clin Microbiol       Date:  1995-02       Impact factor: 5.948

7.  Complete development of Cryptosporidium parvum in a human endometrial carcinoma cell line.

Authors:  K R Rasmussen; N C Larsen; M C Healey
Journal:  Infect Immun       Date:  1993-04       Impact factor: 3.441

8.  Cryptosporidium parvum infection of Caco-2 cell monolayers induces an apical monolayer defect, selectively increases transmonolayer permeability, and causes epithelial cell death.

Authors:  J K Griffiths; R Moore; S Dooley; G T Keusch; S Tzipori
Journal:  Infect Immun       Date:  1994-10       Impact factor: 3.441

9.  Attachment of Cryptosporidium parvum sporozoites to human intestinal epithelial cells.

Authors:  A Joe; R Verdon; S Tzipori; G T Keusch; H D Ward
Journal:  Infect Immun       Date:  1998-07       Impact factor: 3.441

10.  Cryptosporidium parvum DNA replication in cell-free culture.

Authors:  L Zhang; A S Sheoran; G Widmer
Journal:  J Parasitol       Date:  2009-05-23       Impact factor: 1.276
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