| Literature DB >> 19378177 |
Douglas Browning1, Nigel Savery, Annie Kolb, Stephen Busby.
Abstract
Transcription factors interact at promoters to modulate the transcription of genes. This chapter describes three in vitro methods that can be used to monitor their activity: transcript assays, abortive initiation assays, and potassium permanganate footprinting. These techniques have been developed using bacterial systems, and can be used to study the kinetics of transcription initiation, and hence to unravel regulatory mechanisms.Mesh：
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Year: 2009 PMID： 19378177 DOI： 10.1007/978-1-60327-015-1_23
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745