Literature DB >> 19378156

Electrophoretic mobility shift assays for the analysis of DNA-protein interactions.

Manon Gaudreault1, Marie-Eve Gingras, Maryse Lessard, Steeve Leclerc, Sylvain L Guérin.   

Abstract

Electromobility shift assay is a simple, efficient, and rapid method for the study of specific DNA-protein interactions. It relies on the reduction in the electrophoretic mobility conferred to a DNA fragment by an interacting protein. The technique is suitable to qualitative, quantitative, and kinetic analyses. It can also be used to analyze conformational changes.

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Year:  2009        PMID： 19378156     DOI： 10.1007/978-1-60327-015-1_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  8 in total

1.  MEASUREMENT of Protein 53 Diffusion Coefficient in Live HeLa Cells Using Raster Image Correlation Spectroscopy (RICS).

Authors:  Sungmin Hong; Ying-Nai Wang; Hirohito Yamaguchi; Harinibytaraya Sreenivasappa; Chao-Kai Chou; Pei-Hsiang Tsou; Mien-Chie Hung; Jun Kameoka
Journal:  J Biomater Nanobiotechnol       Date:  2010-10

Review 2.  Unravelling the complex genetics of common kidney diseases: from variants to mechanisms.

Authors:  Katie Marie Sullivan; Katalin Susztak
Journal:  Nat Rev Nephrol       Date:  2020-06-08       Impact factor: 28.314

3.  High-throughput screening of prostate cancer risk loci by single nucleotide polymorphisms sequencing.

Authors:  Peng Zhang; Ji-Han Xia; Jing Zhu; Ping Gao; Yi-Jun Tian; Meijun Du; Yong-Chen Guo; Sufyan Suleman; Qin Zhang; Manish Kohli; Lori S Tillmans; Stephen N Thibodeau; Amy J French; James R Cerhan; Li-Dong Wang; Gong-Hong Wei; Liang Wang
Journal:  Nat Commun       Date:  2018-05-22       Impact factor: 14.919

4.  The crystal structure of AcrR from Mycobacterium tuberculosis reveals a one-component transcriptional regulation mechanism.

Authors:  Sung-Min Kang; Do-Hee Kim; Chenglong Jin; Hee-Chul Ahn; Bong-Jin Lee
Journal:  FEBS Open Bio       Date:  2019-08-20       Impact factor: 2.693

5.  Contribution of the Transcription Factors Sp1/Sp3 and AP-1 to Clusterin Gene Expression during Corneal Wound Healing of Tissue-Engineered Human Corneas.

Authors:  Christelle Gross; Gaëtan Le-Bel; Pascale Desjardins; Manel Benhassine; Lucie Germain; Sylvain L Guérin
Journal:  Int J Mol Sci       Date:  2021-11-17       Impact factor: 5.923

6.  Contribution of the STAT Family of Transcription Factors to the Expression of the Serotonin 2B (HTR2B) Receptor in Human Uveal Melanoma.

Authors:  Manel Benhassine; Gaëtan Le-Bel; Sylvain L Guérin
Journal:  Int J Mol Sci       Date:  2022-01-29       Impact factor: 5.923

7.  The Tomato Nucleotide-binding Leucine-rich Repeat Immune Receptor I-2 Couples DNA-binding to Nucleotide-binding Domain Nucleotide Exchange.

Authors:  Stepan Fenyk; Christopher H Dixon; William H Gittens; Philip D Townsend; Gary J Sharples; Lars-Olof Pålsson; Frank L W Takken; Martin J Cann
Journal:  J Biol Chem       Date:  2015-11-24       Impact factor: 5.157

8.  Transcription of the Human 5-Hydroxytryptamine Receptor 2B (HTR2B) Gene Is under the Regulatory Influence of the Transcription Factors NFI and RUNX1 in Human Uveal Melanoma.

Authors:  Manel Benhassine; Sylvain L Guérin
Journal:  Int J Mol Sci       Date:  2018-10-21       Impact factor: 5.923
  8 in total

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