Literature DB >> 19378132

Construction of cell-type specific promoter lentiviruses for optically guiding electrophysiological recordings and for targeted gene delivery.

Aaron M Jasnow1, Donald G Rainnie, Kimberly A Maguschak, Jasmeer P Chhatwal, Kerry J Ressler.   

Abstract

It is often advantageous to identify and alter gene expression of specific cell populations within the brain. Currently, it is not possible to a priori identify specific cell types within the brain of rats for electrophysiological recordings, nor is it possible to routinely alter gene expression in specific cell types within the CNS of a variety of species. Here, we describe a general method for the relatively rapid screening of specific promoter activity in cell culture, in acute brain slice preparations, and in vivo. As an example, we describe the examination of an approximately 3 kb promoter region of the neuroactive peptide cholecystokinin (CCK) compared to the ubiquitous cytomegalovirus (CMV) promoter. We find a high degree of cell-type specificity in vivo using lentiviral approaches in rats and mice.

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Year:  2009        PMID： 19378132      PMCID： PMC5706460          DOI： 10.1007/978-1-59745-559-6_13

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  Gene transfer into neurons from hippocampal slices: comparison of recombinant Semliki Forest Virus, adenovirus, adeno-associated virus, lentivirus, and measles virus.

Authors:  M U Ehrengruber; S Hennou; H Büeler; H Y Naim; N Déglon; K Lundstrom
Journal:  Mol Cell Neurosci       Date:  2001-05       Impact factor: 4.314

2.  Production and purification of lentiviral vectors.

Authors:  Gustavo Tiscornia; Oded Singer; Inder M Verma
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

Review 3.  Lentivirus-mediated gene transfer to the central nervous system: therapeutic and research applications.

Authors:  Liang-Fong Wong; Lucy Goodhead; Christine Prat; Kyriacos A Mitrophanous; Susan M Kingsman; Nicholas D Mazarakis
Journal:  Hum Gene Ther       Date:  2006-01       Impact factor: 5.695

4.  Adeno-associated virus and lentivirus vectors mediate efficient and sustained transduction of cultured mouse and human dorsal root ganglia sensory neurons.

Authors:  J Fleming; S L Ginn; R P Weinberger; T N Trahair; J A Smythe; I E Alexander
Journal:  Hum Gene Ther       Date:  2001-01-01       Impact factor: 5.695

5.  Identification of cell-type-specific promoters within the brain using lentiviral vectors.

Authors:  J P Chhatwal; S E Hammack; A M Jasnow; D G Rainnie; K J Ressler
Journal:  Gene Ther       Date:  2007-01-18       Impact factor: 5.250

6.  Integrated self-inactivating lentiviral vectors produce full-length genomic transcripts competent for encapsidation and integration.

Authors:  Aaron C Logan; Dennis L Haas; Tal Kafri; Donald B Kohn
Journal:  J Virol       Date:  2004-08       Impact factor: 5.103

7.  Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery.

Authors:  R Zufferey; T Dull; R J Mandel; A Bukovsky; D Quiroz; L Naldini; D Trono
Journal:  J Virol       Date:  1998-12       Impact factor: 5.103
  7 in total
  10 in total

Review 1.  Dissecting local circuits in vivo: integrated optogenetic and electrophysiology approaches for exploring inhibitory regulation of cortical activity.

Authors:  Jessica A Cardin
Journal:  J Physiol Paris       Date:  2011-09-19

2.  Amygdala-specific reduction of alpha1-GABAA receptors disrupts the anticonvulsant, locomotor, and sedative, but not anxiolytic, effects of benzodiazepines in mice.

Authors:  Scott A Heldt; Kerry J Ressler
Journal:  J Neurosci       Date:  2010-05-26       Impact factor: 6.167

Review 3.  The role of functional postsynaptic NMDA receptors in the central nucleus of the amygdala in opioid dependence.

Authors:  Michael J Glass
Journal:  Vitam Horm       Date:  2010       Impact factor: 3.421

4.  Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2.

Authors:  Jessica A Cardin; Marie Carlén; Konstantinos Meletis; Ulf Knoblich; Feng Zhang; Karl Deisseroth; Li-Huei Tsai; Christopher I Moore
Journal:  Nat Protoc       Date:  2010-01-21       Impact factor: 13.491

5.  Adrenal MT1 melatonin receptor expression is linked with seasonal variation in social behavior in male Siberian hamsters.

Authors:  Kathleen M Munley; Sohini Dutta; Aaron M Jasnow; Gregory E Demas
Journal:  Horm Behav       Date:  2021-12-15       Impact factor: 3.587

6.  Gephyrin plays a key role in BDNF-dependent regulation of amygdala surface GABAARs.

Authors:  L Mou; B G Dias; H Gosnell; K J Ressler
Journal:  Neuroscience       Date:  2013-10-03       Impact factor: 3.590

7.  In vivo knockdown of GAD67 in the amygdala disrupts fear extinction and the anxiolytic-like effect of diazepam in mice.

Authors:  S A Heldt; L Mou; K J Ressler
Journal:  Transl Psychiatry       Date:  2012-11-13       Impact factor: 6.222

8.  Grin1 receptor deletion within CRF neurons enhances fear memory.

Authors:  Georgette Gafford; Aaron M Jasnow; Kerry J Ressler
Journal:  PLoS One       Date:  2014-10-23       Impact factor: 3.240

9.  The Use of Lentiviral Vectors and Cre/loxP to Investigate the Function of Genes in Complex Behaviors.

Authors:  Scott A Heldt; Kerry J Ressler
Journal:  Front Mol Neurosci       Date:  2009-11-30       Impact factor: 5.639

10.  Optogenetic silencing of a corticotropin-releasing factor pathway from the central amygdala to the bed nucleus of the stria terminalis disrupts sustained fear.

Authors:  A Asok; A Draper; A F Hoffman; J Schulkin; C R Lupica; J B Rosen
Journal:  Mol Psychiatry       Date:  2017-04-25       Impact factor: 15.992
  10 in total

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