| Literature DB >> 19378068 |
Holger Bartsch1, Michael Bachmann.
Abstract
Sera of patients with systemic autoimmune diseases frequently contain autoantibodies to nuclear autoantigens. Immunoblotting of recombinant and native autoantigens is a commonly used technique for the identification and characterization of autoantibody specificities. Here, we describe an easy procedure that facilitates the comparison of antibody specificities by reusing the same immunoblot at least three times in order to detect an abundantly expressed autoantigen in total cellular extracts.Entities:
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Year: 2009 PMID： 19378068 DOI： 10.1007/978-1-59745-542-8_31
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745