Literature DB >> 19377039

Using morpholinos for gene knockdown in Giardia intestinalis.

Meredith L Carpenter1, W Zacheus Cande.   

Abstract

We used translation-blocking morpholinos to reduce protein levels in Giardia intestinalis. Twenty-four hours after electroporation with morpholinos targeting either green fluorescent protein or kinesin-2b, levels of these proteins were reduced by 60%. An epitope-tagged transgene can also be used as a reporter for morpholino efficacy with targets lacking specific antibodies.

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Year:  2009        PMID： 19377039      PMCID： PMC2698301          DOI： 10.1128/EC.00041-09

Source DB:  PubMed          Journal:  Eukaryot Cell        ISSN： 1535-9786


  23 in total

1.  Transcriptional analysis of the glutamate dehydrogenase gene in the primitive eukaryote, Giardia lamblia. Identification of a primordial gene promoter.

Authors:  J Yee; M R Mowatt; P P Dennis; T E Nash
Journal:  J Biol Chem       Date:  2000-04-14       Impact factor: 5.157

Review 2.  Morpholino antisense oligomers: the case for an RNase H-independent structural type.

Authors:  J Summerton
Journal:  Biochim Biophys Acta       Date:  1999-12-10

Review 3.  Biology of Giardia lamblia.

Authors:  R D Adam
Journal:  Clin Microbiol Rev       Date:  2001-07       Impact factor: 26.132

4.  Genome ploidy in different stages of the Giardia lamblia life cycle.

Authors:  R Bernander; J E Palm; S G Svärd
Journal:  Cell Microbiol       Date:  2001-01       Impact factor: 3.715

5.  An ancestral secretory apparatus in the protozoan parasite Giardia intestinalis.

Authors:  Matthias Marti; Attila Regös; Yajie Li; Elisabeth M Schraner; Peter Wild; Norbert Müller; Lea G Knopf; Adrian B Hehl
Journal:  J Biol Chem       Date:  2003-04-23       Impact factor: 5.157

6.  Antigenic variation in Giardia lamblia is regulated by RNA interference.

Authors:  César G Prucca; Ileana Slavin; Rodrigo Quiroga; Eliana V Elías; Fernando D Rivero; Alicia Saura; Pedro G Carranza; Hugo D Luján
Journal:  Nature       Date:  2008-12-11       Impact factor: 49.962

Review 7.  The cytoskeleton of Giardia lamblia.

Authors:  Heidi G Elmendorf; Scott C Dawson; J Michael McCaffery
Journal:  Int J Parasitol       Date:  2003-01       Impact factor: 3.981

8.  Identification and characterization of a novel secretory granule calcium-binding protein from the early branching eukaryote Giardia lamblia.

Authors:  María C Touz; Natalia Gottig; Theodore E Nash; Hugo D Lujan
Journal:  J Biol Chem       Date:  2002-09-26       Impact factor: 5.157

9.  Definition of individual components within the cytoskeleton of Trypanosoma brucei by a library of monoclonal antibodies.

Authors:  A Woods; T Sherwin; R Sasse; T H MacRae; A J Baines; K Gull
Journal:  J Cell Sci       Date:  1989-07       Impact factor: 5.285

10.  Sequence and submolecular localization of the 115-kD accessory subunit of the heterotrimeric kinesin-II (KRP85/95) complex.

Authors:  K P Wedaman; D W Meyer; D J Rashid; D G Cole; J M Scholey
Journal:  J Cell Biol       Date:  1996-02       Impact factor: 10.539
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  28 in total

Review 1.  Life with eight flagella: flagellar assembly and division in Giardia.

Authors:  Scott C Dawson; Susan A House
Journal:  Curr Opin Microbiol       Date:  2010-06-25       Impact factor: 7.934

2.  Nuclear inheritance and genetic exchange without meiosis in the binucleate parasite Giardia intestinalis.

Authors:  Meredith L Carpenter; Zoe June Assaf; Stéphane Gourguechon; W Zacheus Cande
Journal:  J Cell Sci       Date:  2012-02-24       Impact factor: 5.285

3.  Identification of obscure yet conserved actin-associated proteins in Giardia lamblia.

Authors:  Alexander R Paredez; Arash Nayeri; Jennifer W Xu; Jana Krtková; W Zacheus Cande
Journal:  Eukaryot Cell       Date:  2014-04-11

4.  The Critical Role of the Cytoskeleton in the Pathogenesis of Giardia.

Authors:  Christopher Nosala; Scott C Dawson
Journal:  Curr Clin Microbiol Rep       Date:  2015-10-08

5.  The Giardia median body protein is a ventral disc protein that is critical for maintaining a domed disc conformation during attachment.

Authors:  David J Woessner; Scott C Dawson
Journal:  Eukaryot Cell       Date:  2012-01-13

6.  An actin cytoskeleton with evolutionarily conserved functions in the absence of canonical actin-binding proteins.

Authors:  Alexander R Paredez; Zoe June Assaf; David Sept; Ljudmilla Timofejeva; Scott C Dawson; Chung-Ju Rachel Wang; W Z Cande
Journal:  Proc Natl Acad Sci U S A       Date:  2011-03-28       Impact factor: 11.205

Review 7.  Microtubule organelles in Giardia.

Authors:  Kari D Hagen; Shane G McInally; Nicholas D Hilton; Scott C Dawson
Journal:  Adv Parasitol       Date:  2020-02-05       Impact factor: 3.870

8.  Glucosylceramide transferase activity is critical for encystation and viable cyst production by an intestinal protozoan, Giardia lamblia.

Authors:  Tavis L Mendez; Atasi De Chatterjee; Trevor T Duarte; Felipe Gazos-Lopes; Leobarda Robles-Martinez; Debarshi Roy; Jianjun Sun; Rosa A Maldonado; Sukla Roychowdhury; Igor C Almeida; Siddhartha Das
Journal:  J Biol Chem       Date:  2013-04-14       Impact factor: 5.157

Review 9.  Shared and Distinct Mechanisms of Compartmentalized and Cytosolic Ciliogenesis.

Authors:  Tomer Avidor-Reiss; Michel R Leroux
Journal:  Curr Biol       Date:  2015-12-07       Impact factor: 10.834

10.  The Giardia cell cycle progresses independently of the anaphase-promoting complex.

Authors:  Stéphane Gourguechon; Liam J Holt; W Zacheus Cande
Journal:  J Cell Sci       Date:  2013-03-22       Impact factor: 5.285
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