| Literature DB >> 19376609 |
Ma Estrella Santamaría1, Rodrigo Hasbún, Ma José Valera, Mónica Meijón, Luis Valledor, Jose L Rodríguez, Peter E Toorop, Ma Jesús Cañal, Roberto Rodríguez.
Abstract
The relationships between genomic DNA cytosine methylation, histone H4 acetylation and bud dormancy in Castanea sativa are described. Acetylated H4 histone and genomic DNA methylation patterns showed opposite abundance patterns during bud set and bud burst. Increased and decreased methylation levels in the apical buds coincided with bud set and bud burst, respectively. Intermediate axillary buds were characterized by constant levels of DNA methylation during burst of apical buds and reduced fluctuation in DNA methylation throughout the year, which coincided with the absence of macro-morphological changes. Furthermore, acetylated histone H4 (AcH4) levels from apical buds were higher during bud burst than during bud set, as was demonstrated by immunodetection. Results were validated with three additional C. sativa provenances. Thus, global DNA methylation and AcH4 levels showed opposite patterns and coincided with changes in bud dormancy in C. sativa.Entities:
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Year: 2009 PMID： 19376609 DOI： 10.1016/j.jplph.2009.02.014
Source DB: PubMed Journal: J Plant Physiol ISSN： 0176-1617 Impact factor: 3.549