| Literature DB >> 19376554 |
Fu-ying Zheng1, Guo-zhen Lin, Chang-qing Qiu, Ji-zhang Zhou, Xiao-an Cao, Xiao-wei Gong.
Abstract
The gene encoding antigenic site G(1) of bovine ephemeral fever virus (BEFV) was highly expressed in the host cell Escherichia coli. An indirect G(1)-ELISA with the recombinant protein as the coating antigen was established to detect antibodies against BEFV. The result revealed that the optimal concentration of the coated antigen was 0.5 microg/well and the dilution of serum was 1:20. It was optimal that sera with P/N >or= 2.2 were considered positive, P/N <or=2.0 negative, and between 2.0 and 2.2 ambiguous. The G(1)-ELISA method gave a sensitivity of 97.6% and a specificity of 98.6% by testing 590 field serum samples. These results suggest that the G(1)-ELISA may be a good alternative tool for seroepidemiological surveys.Entities:
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Year: 2009 PMID： 19376554 DOI： 10.1016/j.rvsc.2009.03.010
Source DB: PubMed Journal: Res Vet Sci ISSN： 0034-5288 Impact factor: 2.534