Vanessa Montalvo1, Igal Gery. 1. Laboratory of Immunology, National Eye Institute, NIH, Bethesda, MD 20892-1857, USA.
Abstract
PURPOSE: To compare gene expression of pro-apoptotic molecules in the fetal and adult mouse eye. MATERIALS AND METHODS: Eyes were collected from groups of BALB/c mice, at embryonic ages E16 and E18 (fetal) or 6-8 weeks of age (adult). RNA extracted from pooled eyes was analyzed by real-time PCR for transcript levels of pro-apoptotic molecules involved in the intrinsic or extrinsic pathway of apoptosis. RESULTS: Transcripts of intrinsic pathway molecules, Bad, Bak, and Bax, were relatively more highly expressed in the fetal mouse eyes, whereas extrinsic pathway molecules, Fas, FasL, and TRAIL had greater expression in the adult eyes. CONCLUSIONS: Different mechanisms of apoptosis are prominent at the fetal and mature stages of the mouse eye.
PURPOSE: To compare gene expression of pro-apoptotic molecules in the fetal and adult mouse eye. MATERIALS AND METHODS: Eyes were collected from groups of BALB/c mice, at embryonic ages E16 and E18 (fetal) or 6-8 weeks of age (adult). RNA extracted from pooled eyes was analyzed by real-time PCR for transcript levels of pro-apoptotic molecules involved in the intrinsic or extrinsic pathway of apoptosis. RESULTS: Transcripts of intrinsic pathway molecules, Bad, Bak, and Bax, were relatively more highly expressed in the fetalmouse eyes, whereas extrinsic pathway molecules, Fas, FasL, and TRAIL had greater expression in the adult eyes. CONCLUSIONS: Different mechanisms of apoptosis are prominent at the fetal and mature stages of the mouse eye.
Authors: Jayne M Ness; Cary A Harvey; Andreas Strasser; Philippe Bouillet; Barbara J Klocke; Kevin A Roth Journal: Brain Res Date: 2006-06-15 Impact factor: 3.252
Authors: T Lindsten; A J Ross; A King; W X Zong; J C Rathmell; H A Shiels; E Ulrich; K G Waymire; P Mahar; K Frauwirth; Y Chen; M Wei; V M Eng; D M Adelman; M C Simon; A Ma; J A Golden; G Evan; S J Korsmeyer; G R MacGregor; C B Thompson Journal: Mol Cell Date: 2000-12 Impact factor: 17.970
Authors: Paul Hahn; Tullia Lindsten; Gui-Shuang Ying; Jean Bennett; Ann H Milam; Craig B Thompson; Joshua L Dunaief Journal: Invest Ophthalmol Vis Sci Date: 2003-08 Impact factor: 4.799