| Literature DB >> 19371953 |
Seung-Kiel Park1, Huihong Qiao, Michael A Beaven.
Abstract
Our studies in the RBL-2H3 mast cell line suggest that responses to antigen (Ag) are negatively modulated through upregulation of Src-like adaptor protein (SLAP). Ag stimulation of RBL-2H3 cells leads to increased levels of SLAP (but not SLAP2) transcripts and protein over a period of several hours. The effects of pharmacologic inhibitors indicate that the upregulation of SLAP is dependent on multiple signaling pathways. Knockdown of SLAP with anti-SLAP siRNA is associated with enhanced phosphorylation of Syk, the linker for activation of T cells (LAT), phospholipase C gamma, MAP kinases, and various transcription factors. Production of IL-3 and MCP-1, but not degranulation, is also enhanced. The upregulation of SLAP may thus serve to limit the duration of cytokine production in Ag-stimulated cells.Entities:
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Year: 2009 PMID： 19371953 PMCID： PMC2684944 DOI： 10.1016/j.molimm.2009.03.013
Source DB: PubMed Journal: Mol Immunol ISSN： 0161-5890 Impact factor: 4.407