Literature DB >> 19365792

Autoinduction of protein expression.

Brian G Fox1, Paul G Blommel.   

Abstract

This unit contains protocols for the use of lactose-derived autoinduction in Escherichia coli. The protocols allow for reproducible expression trials to be undertaken with minimal user intervention. A basic protocol covers production of unlabeled proteins for functional studies. Alternate protocols for selenomethionine labeling for X-ray structural studies, and multi-well plate growth for screening and optimization are also included.

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Year:  2009        PMID： 19365792      PMCID： PMC5602607          DOI： 10.1002/0471140864.ps0523s56

Source DB:  PubMed          Journal:  Curr Protoc Protein Sci        ISSN： 1934-3655


  30 in total

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Journal:  J Struct Biol       Date:  2003-04       Impact factor: 2.867

2.  Macromolecular crystallization with microfluidic free-interface diffusion.

Authors:  Brent Segelke
Journal:  Expert Rev Proteomics       Date:  2005-04       Impact factor: 3.940

Review 3.  High-throughput screening of biocatalytic activity: applications in drug discovery.

Authors:  R Anand Kumar; Douglas S Clark
Journal:  Curr Opin Chem Biol       Date:  2006-03-07       Impact factor: 8.822

4.  A Protein Structure Initiative approach to expression, purification, and in situ delivery of human cytochrome b5 to membrane vesicles.

Authors:  Pablo Sobrado; Michael A Goren; Declan James; Carissa K Amundson; Brian G Fox
Journal:  Protein Expr Purif       Date:  2007-12-15       Impact factor: 1.650

5.  An improved strategy for high-level production of TEV protease in Escherichia coli and its purification and characterization.

Authors:  Lei Fang; Kun-Zhi Jia; Ya-Lan Tang; Ding-Yuan Ma; Mei Yu; Zi-Chun Hua
Journal:  Protein Expr Purif       Date:  2006-07-12       Impact factor: 1.650

6.  The codon Adaptation Index--a measure of directional synonymous codon usage bias, and its potential applications.

Authors:  P M Sharp; W H Li
Journal:  Nucleic Acids Res       Date:  1987-02-11       Impact factor: 16.971

7.  Spinach holo-acyl carrier protein: overproduction and phosphopantetheinylation in Escherichia coli BL21(DE3), in vitro acylation, and enzymatic desaturation of histidine-tagged isoform I.

Authors:  J A Broadwater; B G Fox
Journal:  Protein Expr Purif       Date:  1999-04       Impact factor: 1.650

8.  Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination.

Authors:  Robert C Tyler; Hassan K Sreenath; Shanteri Singh; David J Aceti; Craig A Bingman; John L Markley; Brian G Fox
Journal:  Protein Expr Purif       Date:  2005-04       Impact factor: 1.650

9.  Application of fed-batch fermentation to the preparation of isotopically labeled or selenomethionyl-labeled proteins.

Authors:  J M Studts; B G Fox
Journal:  Protein Expr Purif       Date:  1999-06       Impact factor: 1.650

10.  Fluorescence anisotropy assay for proteolysis of specifically labeled fusion proteins.

Authors:  Paul G Blommel; Brian G Fox
Journal:  Anal Biochem       Date:  2005-01-01       Impact factor: 3.365
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  33 in total

1.  Mutational analysis of quinolone resistance protein QnrB1.

Authors:  George A Jacoby; Marian A Corcoran; Debra M Mills; Caitlin M Griffin; David C Hooper
Journal:  Antimicrob Agents Chemother       Date:  2013-08-26       Impact factor: 5.191

2.  UDP-galactopyranose mutases from Leishmania species that cause visceral and cutaneous leishmaniasis.

Authors:  Isabel O Fonseca; Karina Kizjakina; Pablo Sobrado
Journal:  Arch Biochem Biophys       Date:  2013-09-03       Impact factor: 4.013

3.  Prion Protein Prolines 102 and 105 and the Surrounding Lysine Cluster Impede Amyloid Formation.

Authors:  Allison Kraus; Kelsie J Anson; Lynne D Raymond; Craig Martens; Bradley R Groveman; David W Dorward; Byron Caughey
Journal:  J Biol Chem       Date:  2015-07-14       Impact factor: 5.157

4.  Active site and laminarin binding in glycoside hydrolase family 55.

Authors:  Christopher M Bianchetti; Taichi E Takasuka; Sam Deutsch; Hannah S Udell; Eric J Yik; Lai F Bergeman; Brian G Fox
Journal:  J Biol Chem       Date:  2015-03-09       Impact factor: 5.157

5.  A conserved domain important for association of eukaryotic J-protein co-chaperones Jjj1 and Zuo1 with the ribosome.

Authors:  Lindsey A Kaschner; Ruchika Sharma; Om Kumar Shrestha; Alison E Meyer; Elizabeth A Craig
Journal:  Biochim Biophys Acta       Date:  2015-01-30

6.  A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis.

Authors:  Evan M Glasgow; Elias I Kemna; Craig A Bingman; Nicole L Ing; Kai Deng; Christopher M Bianchetti; Taichi E Takasuka; Trent R Northen; Brian G Fox
Journal:  J Biol Chem       Date:  2020-10-16       Impact factor: 5.157

7.  An unprecedented NADPH domain conformation in lysine monooxygenase NbtG provides insights into uncoupling of oxygen consumption from substrate hydroxylation.

Authors:  Claudia Binda; Reeder M Robinson; Julia S Martin Del Campo; Nicholas D Keul; Pedro J Rodriguez; Howard H Robinson; Andrea Mattevi; Pablo Sobrado
Journal:  J Biol Chem       Date:  2015-03-23       Impact factor: 5.157

8.  Mitochondrial ADCK3 employs an atypical protein kinase-like fold to enable coenzyme Q biosynthesis.

Authors:  Jonathan A Stefely; Andrew G Reidenbach; Arne Ulbrich; Krishnadev Oruganty; Brendan J Floyd; Adam Jochem; Jaclyn M Saunders; Isabel E Johnson; Catherine E Minogue; Russell L Wrobel; Grant E Barber; David Lee; Sheng Li; Natarajan Kannan; Joshua J Coon; Craig A Bingman; David J Pagliarini
Journal:  Mol Cell       Date:  2014-12-11       Impact factor: 17.970

9.  Discovery of an L-fucono-1,5-lactonase from cog3618 of the amidohydrolase superfamily.

Authors:  Merlin Eric Hobbs; Matthew Vetting; Howard J Williams; Tamari Narindoshvili; Devon M Kebodeaux; Brandan Hillerich; Ronald D Seidel; Steven C Almo; Frank M Raushel
Journal:  Biochemistry       Date:  2012-12-20       Impact factor: 3.162

10.  Improved expression and purification of sigma 1 receptor fused to maltose binding protein by alteration of linker sequence.

Authors:  Katarzyna A Gromek; Hannah R Meddaugh; Russell L Wrobel; Fabian P Suchy; Craig A Bingman; John G Primm; Brian G Fox
Journal:  Protein Expr Purif       Date:  2013-04-03       Impact factor: 1.650
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