Literature DB >> 19365789

Imaging protein-protein interactions by Förster resonance energy transfer (FRET) microscopy in live cells.

Joseph A Brzostowski1, Tobias Meckel, Jiang Hong, Alice Chen, Tian Jin.   

Abstract

This unit describes an acceptor-sensitized emission FRET method using a confocal microscope for image acquisition. In contrast to acceptor photobleaching, which is an end-point assay that destroys the acceptor fluorophore, the sensitized emission method is amenable for FRET measurements in live cells and can be used to measure changes in FRET efficiency over time. The purpose of this unit is to provide a basic starting point for understanding and performing the sensitized emission method with a simple teaching tool for live-cell imaging. References that discuss the vagaries of acquiring and analyzing FRET between individually tagged molecules are provided.

Entities:  

Mesh：

Substances：

Year:  2009        PMID： 19365789      PMCID： PMC3568943          DOI： 10.1002/0471140864.ps1905s56

Source DB:  PubMed          Journal:  Curr Protoc Protein Sci        ISSN： 1934-3655


  16 in total

1.  Receptor-mediated activation of heterotrimeric G-proteins in living cells.

Authors:  C Janetopoulos; T Jin; P Devreotes
Journal:  Science       Date:  2001-03-23       Impact factor: 47.728

2.  Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin.

Authors:  A Miyawaki; J Llopis; R Heim; J M McCaffery; J A Adams; M Ikura; R Y Tsien
Journal:  Nature       Date:  1997-08-28       Impact factor: 49.962

3.  Photobleaching-corrected FRET efficiency imaging of live cells.

Authors:  Tomasz Zal; Nicholas R J Gascoigne
Journal:  Biophys J       Date:  2004-06       Impact factor: 4.033

4.  Correcting confocal acquisition to optimize imaging of fluorescence resonance energy transfer by sensitized emission.

Authors:  Jacco van Rheenen; Michiel Langeslag; Kees Jalink
Journal:  Biophys J       Date:  2004-04       Impact factor: 4.033

5.  An improved cyan fluorescent protein variant useful for FRET.

Authors:  Mark A Rizzo; Gerald H Springer; Butch Granada; David W Piston
Journal:  Nat Biotechnol       Date:  2004-02-29       Impact factor: 54.908

6.  A fluorescent indicator for visualizing cAMP-induced phosphorylation in vivo.

Authors:  Y Nagai; M Miyazaki; R Aoki; T Zama; S Inouye; K Hirose; M Iino; M Hagiwara
Journal:  Nat Biotechnol       Date:  2000-03       Impact factor: 54.908

7.  The initiation of antigen-induced B cell antigen receptor signaling viewed in living cells by fluorescence resonance energy transfer.

Authors:  Pavel Tolar; Hae Won Sohn; Susan K Pierce
Journal:  Nat Immunol       Date:  2005-10-02       Impact factor: 25.606

8.  Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy.

Authors:  G W Gordon; G Berry; X H Liang; B Levine; B Herman
Journal:  Biophys J       Date:  1998-05       Impact factor: 4.033

9.  Reliable and global measurement of fluorescence resonance energy transfer using fluorescence microscopes.

Authors:  Z Xia; Y Liu
Journal:  Biophys J       Date:  2001-10       Impact factor: 4.033

10.  Quantitative imaging of single live cells reveals spatiotemporal dynamics of multistep signaling events of chemoattractant gradient sensing in Dictyostelium.

Authors:  Xuehua Xu; Martin Meier-Schellersheim; Xuanmao Jiao; Lauren E Nelson; Tian Jin
Journal:  Mol Biol Cell       Date:  2004-11-24       Impact factor: 4.138
View more
  6 in total

1.  n-3 polyunsaturated fatty acids suppress CD4(+) T cell proliferation by altering phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] organization.

Authors:  Tim Y Hou; Rola Barhoumi; Yang-Yi Fan; Gonzalo M Rivera; Rami N Hannoush; David N McMurray; Robert S Chapkin
Journal:  Biochim Biophys Acta       Date:  2015-10-23

2.  A Gβγ effector, ElmoE, transduces GPCR signaling to the actin network during chemotaxis.

Authors:  Jianshe Yan; Vassil Mihaylov; Xuehua Xu; Joseph A Brzostowski; Hongyan Li; Lunhua Liu; Timothy D Veenstra; Carole A Parent; Tian Jin
Journal:  Dev Cell       Date:  2012-01-17       Impact factor: 12.270

3.  3D/4D functional imaging of tumor-associated proteolysis: impact of microenvironment.

Authors:  Kamiar Moin; Mansoureh Sameni; Bernadette C Victor; Jennifer M Rothberg; Raymond R Mattingly; Bonnie F Sloane
Journal:  Methods Enzymol       Date:  2012       Impact factor: 1.600

4.  Nanoscopy of the cellular response to hypoxia by means of fluorescence resonance energy transfer (FRET) and new FRET software.

Authors:  Christoph Wotzlaw; Silke Gneuss; Rebecca Konietzny; Joachim Fandrey
Journal:  PMC Biophys       Date:  2010-03-05

5.  Fluorescence resonance energy transfer-based intracellular assay for the conformation of hepatitis C virus drug target NS5A.

Authors:  Dipankar Bhattacharya; Israrul H Ansari; Andrew Mehle; Rob Striker
Journal:  J Virol       Date:  2012-05-23       Impact factor: 5.103

6.  TRPC3 contributes to regulation of cardiac contractility and arrhythmogenesis by dynamic interaction with NCX1.

Authors:  Bernhard Doleschal; Uwe Primessnig; Gerald Wölkart; Stefan Wolf; Michaela Schernthaner; Michaela Lichtenegger; Toma N Glasnov; C Oliver Kappe; Bernd Mayer; Gudrun Antoons; Frank Heinzel; Michael Poteser; Klaus Groschner
Journal:  Cardiovasc Res       Date:  2015-01-28       Impact factor: 13.081
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.