| Literature DB >> 19364507 |
Hana Ra1, Hyun-Lim Kim, Han-Woong Lee, Yang-Hee Kim.
Abstract
Depletion of intracellular zinc with N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) induces protein synthesis-dependent apoptosis. In this study, we examined the requirement for p53 as an upstream transcription factor in TPEN-induced neuronal apoptosis. Chemical or genetic blockade of p53 markedly attenuated TPEN-induced neuronal apoptosis, while the stability and activity of p53 were increased by TPEN. In addition, expression of proapoptotic genes, PUMA and NOXA, and activation of caspase-11 were increased by TPEN in a p53-dependent manner. Inhibition of p53 blocked cytochrome C release from mitochondria to cytosol and prevented caspase-3 activation. Therefore, p53 may be an essential regulatory factor for TPEN-induced neuronal apoptosis.Entities:
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Year: 2009 PMID： 19364507 DOI： 10.1016/j.febslet.2009.04.008
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124