M Orazizadeh1, M Hashemitabar, L Khorsandi. 1. Department of Anatomical Sciences, Faculty of Medicine, Ahwaz Jundi-Shapour University of Medical Sciences, Ahwaz, Iran.
Abstract
BACKGROUND: Apoptosis involves in testicular germ cell loss in animals and humans, and plays an important role in male fertility. Previous studies have reported neuroprotective and antiapoptotic effects of minocycline. AIM: In this study the protective effect of Minocycline on testicular germ cell apoptosis arising from dexamethasone (Dex) has been evaluated. Dex is a widely used as a glucocorticoid (GC) agent that its apoptotic effect has been shown. MATERIALS AND METHODS: Experimental groups of 8 male mice received one of the following treatments daily for 7 days: 100 mg/kg Minocycline, 7 mg/kg Dex and 7 mg/kg Dex +100 mg/kg Minocycline. Control group was treated with 0.5 ml saline given orally for a week. Then the mice were sacrificed, and their testes processed for assessment of germ cell apoptosis (TUNEL method), the quality of spermatogenesis (Johnsen score system) and testicular sperm counts. RESULTS: Germ cell apoptosis were significantly increased in Dex treated mice compared with control (P < 0.01). Spermatogenesis and the number of sperms head were significantly reduced in Dex treated mice compared with those of the control group (P < 0.01). Treatment with Dex and Minocycline resulted in an inhibition effect on germ cell apoptosis and a significant increase in the Johnsen score and the number of head sperm compared with Dex treated mice (P < 0.05). CONCLUSION: The application of Minocycline may serve as a beneficial medication to protect germ cells against apoptosis.
BACKGROUND: Apoptosis involves in testicular germ cell loss in animals and humans, and plays an important role in male fertility. Previous studies have reported neuroprotective and antiapoptotic effects of minocycline. AIM: In this study the protective effect of Minocycline on testicular germ cell apoptosis arising from dexamethasone (Dex) has been evaluated. Dex is a widely used as a glucocorticoid (GC) agent that its apoptotic effect has been shown. MATERIALS AND METHODS: Experimental groups of 8 male mice received one of the following treatments daily for 7 days: 100 mg/kg Minocycline, 7 mg/kg Dex and 7 mg/kg Dex +100 mg/kg Minocycline. Control group was treated with 0.5 ml saline given orally for a week. Then the mice were sacrificed, and their testes processed for assessment of germ cell apoptosis (TUNEL method), the quality of spermatogenesis (Johnsen score system) and testicular sperm counts. RESULTS: Germ cell apoptosis were significantly increased in Dex treated mice compared with control (P < 0.01). Spermatogenesis and the number of sperms head were significantly reduced in Dex treated mice compared with those of the control group (P < 0.01). Treatment with Dex and Minocycline resulted in an inhibition effect on germ cell apoptosis and a significant increase in the Johnsen score and the number of head sperm compared with Dex treated mice (P < 0.05). CONCLUSION: The application of Minocycline may serve as a beneficial medication to protect germ cells against apoptosis.
Authors: P Surampudi; I Chang; Y Lue; T Doumit; Y Jia; V Atienza; P Y Liu; R S Swerdloff; C Wang Journal: Andrology Date: 2015-04-16 Impact factor: 3.842
Authors: Ivan Delgado-Enciso; Alejandro García-Rivera; M Violeta M Madrigal-Pérez; Alejandrina Rodriguez-Hernandez; Agustin Lugo-Radillo; Hector R Galvan-Salazar; Alejandro D Soriano-Hernández; Fernando Gómez-Tapia; Rafael Martinez-Martinez; Laura L Valdez-Velazquez; Oscar A Newton-Sanchez; Rafael Gonzalez-Alvarez; Iram P Rodriguez-Sanchez; Valery Melnikov; Agustin Lara-Esqueda; Jose Guzman-Esquivel Journal: Int Urol Nephrol Date: 2013-11-28 Impact factor: 2.370