OBJECTIVES: Dendritic cells (DCs) are among the first cells to encounter HIV after mucosal exposure and can bind virus via C-type lectin receptors (CLRs). Here, we characterized the distribution of various DC subtypes and the density of the CLRs, DC-SIGN, langerin, and mannose receptor in the ectocervix of HIV-seronegative women with low- and high-risk behavior for acquiring HIV. MATERIAL AND METHODS: Cryosections from ectocervical biopsies, collected from sexually active low-risk healthy HIV immunoglobulin G-negative women (n = 10) and HIV immunoglobulin G-negative commercial sex workers (n = 8), were assessed by computerized image analysis. RESULTS: We identified various distinct DC populations. CD11c(-)CD1a(+)langerin(+) cells were localized in the epithelium, whereas CD11c(+)CD1a(-)DC-SIGN and CD11c(-)CD1a(-)CD68(+)DC-SIGN(+)mannose receptor(+) cells were restricted to the lamina propria of the ectocervix. CD123(+) cells were found at low incidence and did not express any of the investigated CLRs. The density of CLR expression was significantly higher in the high-risk as compared with the low-risk women. CONCLUSIONS: The superficial and abundant presence of potential HIV target cells makes the ectocervix a likely site for HIV transmission. The detected variations in density and localization of potential HIV receptors should be considered when developing topical prophylactic measures.
OBJECTIVES: Dendritic cells (DCs) are among the first cells to encounter HIV after mucosal exposure and can bind virus via C-type lectin receptors (CLRs). Here, we characterized the distribution of various DC subtypes and the density of the CLRs, DC-SIGN, langerin, and mannose receptor in the ectocervix of HIV-seronegative women with low- and high-risk behavior for acquiring HIV. MATERIAL AND METHODS: Cryosections from ectocervical biopsies, collected from sexually active low-risk healthy HIV immunoglobulin G-negative women (n = 10) and HIV immunoglobulin G-negative commercial sex workers (n = 8), were assessed by computerized image analysis. RESULTS: We identified various distinct DC populations. CD11c(-)CD1a(+)langerin(+) cells were localized in the epithelium, whereas CD11c(+)CD1a(-)DC-SIGN and CD11c(-)CD1a(-)CD68(+)DC-SIGN(+)mannose receptor(+) cells were restricted to the lamina propria of the ectocervix. CD123(+) cells were found at low incidence and did not express any of the investigated CLRs. The density of CLR expression was significantly higher in the high-risk as compared with the low-risk women. CONCLUSIONS: The superficial and abundant presence of potential HIV target cells makes the ectocervix a likely site for HIV transmission. The detected variations in density and localization of potential HIV receptors should be considered when developing topical prophylactic measures.
Authors: Taha Hirbod; Robert C Bailey; Kawango Agot; Stephen Moses; Jeckoniah Ndinya-Achola; Ruth Murugu; Jan Andersson; Jakob Nilsson; Kristina Broliden Journal: Am J Pathol Date: 2010-04-15 Impact factor: 4.307
Authors: M Peressin; V Holl; S Schmidt; T Decoville; D Mirisky; A Lederle; M Delaporte; K Xu; A M Aubertin; C Moog Journal: J Virol Date: 2010-11-17 Impact factor: 5.103
Authors: Emily Bond; Frank Liang; Kerrie J Sandgren; Anna Smed-Sörensen; Peter Bergman; Susanna Brighenti; William C Adams; Senait A Betemariam; Molebogeng X Rangaka; Christoph Lange; Robert J Wilkinson; Jan Andersson; Karin Loré Journal: J Invest Dermatol Date: 2011-08-18 Impact factor: 8.551
Authors: Lydia Guadalupe Rivera-Morales; Paulo Lopez-Guillen; Jose Manuel Vazquez-Guillen; Gerardo C Palacios-Saucedo; Adrian G Rosas-Taraco; Antonio Ramirez-Pineda; Patricia Irene Amaya-Garcia; Cristina Rodriguez-Padilla Journal: Open AIDS J Date: 2012-10-05