| Literature DB >> 19361556 |
Chun-Lei Yuan1, Jun-Fa Xu, Jing Tong, Heng Yang, Feng-Rong He, Quan Gong, Ping Xiong, Lihua Duan, Min Fang, Zheng Tan, Yong Xu, Yi-Fa Chen, Fang Zheng, Fei-Li Gong.
Abstract
B7-H4, a recently discovered member of B7 family, can negatively regulate T cell responses. However, it is not clear whether B7-H4 negatively function in cell transplantation. In this study we investigated the immunosuppressive effect of B7-H4 on beta-cell transplantation. An insulinoma cell line, NIT-1, transfected with B7-H4 (B7-H4-NIT) was established, and transplanted to diabetic C57BL/6 mice by intraperitoneal injection. Proliferation assay of splenocytes in vitro showed that B7-H4-NIT suppressed alloreactive T cell activation. The proportion of IFN-gamma-producing cells in recipient spleen was significantly reduced and the number of Treg cells was upregulated in B7-H4-NIT group compared to the control, EGFP-NIT. The expression of mRNA coding IFN-gamma was lower but that of IL-4 was higher in B7-H4-NIT transplanted recipients than in the control animals. The results of ELISA also revealed the same trends. Diabetic mice reached normalglycemic quickly and gained weight after transplantation of B7-H4-NIT. More importantly, the survival time for recipients transplanted with B7-H4-NIT cells was significantly longer than that with EGFP-NIT cells. These results indicate that B7-H4 transfection prolongs beta-cell graft survival.Entities:
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Year: 2009 PMID： 19361556 DOI： 10.1016/j.trim.2009.03.007
Source DB: PubMed Journal: Transpl Immunol ISSN： 0966-3274 Impact factor: 1.708