BACKGROUND: Pesticide-formulating industries are contaminating the environment through various activities. Bioremediation is the best method for decontamination, as chemical and physical methods are not only costly but also not very effective in open field systems. In the present study, in situ bioremediation of organochlorine-contaminated soil was demonstrated by combined biostimulation and bioaugmentation strategies, followed by evaluation using a molecular method. RESULTS: Three parameters were monitored: microbial biomass (colony-forming units (CFU) g(-1) soil), residual pesticides after treatment and catabolic genes from microcosm soil. Both the biostimulation and the bioaugmentation treatments showed an initial lag phase of 80 days towards colony-forming units. Gas chromatography of soil samples showed that concentrations of residual pesticides in the soil declined by up to 85-90% after 80 days, indicating their utilisation with time. On dot-blot hybridisation of the total DNA from the same soil samples, it was observed that catabolic genes tfdC (catechol 1,2-dioxygenase) and cm genes (chlorophenol monoxygenase) were predominant, whereas other catabolic genes such as catechol 2,3-dioxygenase (xylE) were negligible. CONCLUSION: The strategy of in situ bioremediation and its evaluation by gene probe and also by conventional methods was demonstrated for organochlorine-pesticide-contaminated soil in open microcosms. It showed that bioaugmentation along with biostimulation was effective, although initial acclimatization for a period of almost 2-3 months was required in the open field systems. Copyright (c) 2009 Society of Chemical Industry.
BACKGROUND: Pesticide-formulating industries are contaminating the environment through various activities. Bioremediation is the best method for decontamination, as chemical and physical methods are not only costly but also not very effective in open field systems. In the present study, in situ bioremediation of organochlorine-contaminated soil was demonstrated by combined biostimulation and bioaugmentation strategies, followed by evaluation using a molecular method. RESULTS: Three parameters were monitored: microbial biomass (colony-forming units (CFU) g(-1) soil), residual pesticides after treatment and catabolic genes from microcosm soil. Both the biostimulation and the bioaugmentation treatments showed an initial lag phase of 80 days towards colony-forming units. Gas chromatography of soil samples showed that concentrations of residual pesticides in the soil declined by up to 85-90% after 80 days, indicating their utilisation with time. On dot-blot hybridisation of the total DNA from the same soil samples, it was observed that catabolic genes tfdC (catechol 1,2-dioxygenase) and cm genes (chlorophenol monoxygenase) were predominant, whereas other catabolic genes such as catechol 2,3-dioxygenase (xylE) were negligible. CONCLUSION: The strategy of in situ bioremediation and its evaluation by gene probe and also by conventional methods was demonstrated for organochlorine-pesticide-contaminated soil in open microcosms. It showed that bioaugmentation along with biostimulation was effective, although initial acclimatization for a period of almost 2-3 months was required in the open field systems. Copyright (c) 2009 Society of Chemical Industry.