Literature DB >> 1935982

Purification and biochemical characterization of dog gastric lipase.

F Carrière1, H Moreau, V Raphel, R Laugier, C Benicourt, J L Junien, R Verger.   

Abstract

A lipase was found to be present in dog stomach which appeared to be more abundant in the fundic than in the pyloric mucosa. Dog gastric lipase was extracted by soaking the gastric tissue and further purified after cation exchange, anion exchange and gel-filtration using fast protein liquid chromatography. The amino-acid composition, N-terminal amino-acid sequence, substrate specificity, interfacial and kinetic behavior and inactivation by sulfhydryl reagents were determined and compared with those of human and rabbit gastric lipases. We report for the first time that a gastric lipase is 13 times more active on long-chain than on short-chain triacylglycerols at pH 4.0, reaching a maximal specific activity of 950 U/mg on Intralipide emulsion.

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Year:  1991        PMID： 1935982     DOI： 10.1111/j.1432-1033.1991.tb16346.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  11 in total

1.  A broad pH range indicator-based spectrophotometric assay for true lipases using tributyrin and tricaprylin.

Authors:  María de Los Angeles Camacho-Ruiz; Juan Carlos Mateos-Díaz; Frédéric Carrière; Jorge A Rodriguez
Journal:  J Lipid Res       Date:  2015-03-07       Impact factor: 5.922

Review 2.  Lipids in the Stomach - Implications for the Evaluation of Food Effects on Oral Drug Absorption.

Authors:  Mirko Koziolek; Frédéric Carrière; Christopher J H Porter
Journal:  Pharm Res       Date:  2018-02-08       Impact factor: 4.200

3.  Toward the establishment of standardized in vitro tests for lipid-based formulations, part 6: effects of varying pancreatin and calcium levels.

Authors:  Philip Sassene; Karen Kleberg; Hywel D Williams; Jean-Claude Bakala-N'Goma; Frédéric Carrière; Marilyn Calderone; Vincent Jannin; Annabel Igonin; Anette Partheil; Delphine Marchaud; Eduardo Jule; Jan Vertommen; Mario Maio; Ross Blundell; Hassan Benameur; Christopher J H Porter; Colin W Pouton; Anette Müllertz
Journal:  AAPS J       Date:  2014-10-02       Impact factor: 4.009

4.  Synthesis and kinetic evaluation of cyclophostin and cyclipostins phosphonate analogs as selective and potent inhibitors of microbial lipases.

Authors:  Vanessa Point; Raj K Malla; Sadia Diomande; Benjamin P Martin; Vincent Delorme; Frederic Carriere; Stephane Canaan; Nigam P Rath; Christopher D Spilling; Jean-François Cavalier
Journal:  J Med Chem       Date:  2012-11-07       Impact factor: 7.446

5.  Development and analytical validation of an enzyme linked immunosorbent assay for the measurement of canine gastric lipase immunoreactivity in serum.

Authors:  Jörg M Steiner; David A Williams
Journal:  Can J Vet Res       Date:  2004-07       Impact factor: 1.310

6.  Toward the establishment of standardized in vitro tests for lipid-based formulations. 5. Lipolysis of representative formulations by gastric lipase.

Authors:  Jean-Claude Bakala-N'Goma; Hywel D Williams; Philip J Sassene; Karen Kleberg; Marilyn Calderone; Vincent Jannin; Annabel Igonin; Anette Partheil; Delphine Marchaud; Eduardo Jule; Jan Vertommen; Mario Maio; Ross Blundell; Hassan Benameur; Anette Müllertz; Colin W Pouton; Christopher J H Porter; Frédéric Carrière
Journal:  Pharm Res       Date:  2014-10-07       Impact factor: 4.200

7.  In vitro digestion of the self-emulsifying lipid excipient Labrasol(®) by gastrointestinal lipases and influence of its colloidal structure on lipolysis rate.

Authors:  Sylvie Fernandez; Vincent Jannin; Stéphanie Chevrier; Yann Chavant; Frédéric Demarne; Frédéric Carrière
Journal:  Pharm Res       Date:  2013-05-02       Impact factor: 4.200

8.  Inhibition studies on calf pregastric esterase: the enzyme has no functional thiol group.

Authors:  M Y Timmermans; G Reekmans; H J Teuchy; L P Kupers
Journal:  Biochem J       Date:  1996-03-15       Impact factor: 3.857

9.  Cloning, phylogenetic analysis and 3D modeling of a putative lysosomal acid lipase from the camel, Camelus dromedarius.

Authors:  Farid Shokry Ataya
Journal:  Molecules       Date:  2012-08-30       Impact factor: 4.411

10.  Pancreatic lipase-related protein 2 digests fats in human milk and formula in concert with gastric lipase and carboxyl ester lipase.

Authors:  Karin Johnson; Leah Ross; Rita Miller; Xunjun Xiao; Mark E Lowe
Journal:  Pediatr Res       Date:  2013-06-03       Impact factor: 3.756
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