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Literature DB >> 19359

Fungicidal properties of a chymotrypsin-like cationic protein from human neutrophils: adsorption to Candida parapsilosis.

Abstract

Human neutrophils contain a chymotrypsin-like cationic protein (CLCP) that binds to the surface of Candida parapsilosis and is preferentially adsorbed by yeasts mixed with unfractionated extracts of neutrophil granules. Adsorption of CLCP to opsonized or nonopsonized yeasts was rapid at pH 4 through 8. Irreversible inhibition of the enzymatic site of CLCP by phenylmethylsulfonylfluoride or N-acetyl-Ala-Ala-Phe-chloromethyl ketone did not affect its adsorption by yeast. Adsorption, highly sensitive to ionic strength, was abrogated by 0.15 M KCl. The number of CLCP molecules adsorbed per yeast cell and the loss of colony-forming units are described by an exponential relationship.

Entities: Chemical Species

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Year: 1977 PMID： 19359 PMCID： PMC421132 DOI： 10.1128/iai.17.2.382-388.1977

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

18 in total

1. A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin.

Authors: B C HUMMEL
Journal: Can J Biochem Physiol Date: 1959-12

2. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.

Authors: R A REISFELD; U J LEWIS; D E WILLIAMS
Journal: Nature Date: 1962-07-21 Impact factor: 49.962

3. The role of superoxide anion generation in phagocytic bactericidal activity. Studies with normal and chronic granulomatous disease leukocytes.

Authors: R B Johnston; B B Keele; H P Misra; J E Lehmeyer; L S Webb; R L Baehner; K V RaJagopalan
Journal: J Clin Invest Date: 1975-06 Impact factor: 14.808

4. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

5. Antibacterial activity of cationic proteins from human granulocytes.

Authors: H Odeberg; I Olsson
Journal: J Clin Invest Date: 1975-11 Impact factor: 14.808

6. Role of the superoxide anion in the myeloperoxidase-mediated antimicrobial system.

Authors: S J Klebanoff
Journal: J Biol Chem Date: 1974-06-25 Impact factor: 5.157

7. Cationic proteins of human granulocytes. II. Separation of the cationic proteins of the granules of leukemic myeloid cells.

Authors: I Olsson; P Venge
Journal: Blood Date: 1974-08 Impact factor: 22.113

8. Human leukocyte granule elastase: rapid isolation and characterization.

Authors: R J Baugh; J Travis
Journal: Biochemistry Date: 1976-02-24 Impact factor: 3.162

9. Nonoxidative fungicidal mechanisms of mammalian granulocytes: demonstration of components with candidacidal activity in human, rabbit, and guinea pig leukocytes.

Authors: R I Lehrer; K M Ladra; R B Hake
Journal: Infect Immun Date: 1975-06 Impact factor: 3.441

10. Antibody-dependent cell-mediated cytotoxicity against IBR-infected bovine kidney cells by ruminant neutrophils: the role of lysosomal cationic protein.

Authors: K J Thorne; J M Norman; S F Haydock; D A Lammas; P H Duffus
Journal: Immunology Date: 1984-10 Impact factor: 7.397

Fungicidal properties of a chymotrypsin-like cationic protein from human neutrophils: adsorption to Candida parapsilosis.

1. A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin.

2. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.

3. The role of superoxide anion generation in phagocytic bactericidal activity. Studies with normal and chronic granulomatous disease leukocytes.

4. Protein measurement with the Folin phenol reagent.

5. Antibacterial activity of cationic proteins from human granulocytes.

6. Role of the superoxide anion in the myeloperoxidase-mediated antimicrobial system.

7. Cationic proteins of human granulocytes. II. Separation of the cationic proteins of the granules of leukemic myeloid cells.

8. Human leukocyte granule elastase: rapid isolation and characterization.

9. Nonoxidative fungicidal mechanisms of mammalian granulocytes: demonstration of components with candidacidal activity in human, rabbit, and guinea pig leukocytes.

10. Myeloperoxidase-halide-hydrogen peroxide antibacterial system.

1. Candidacidal activities of proteins partially purified from rat epidermis.

2. Antibiotic proteins of human polymorphonuclear leukocytes.

3. In vivo degradation of gonococcal outer membrane proteins within human leukocyte phagolysosomes.

Review 4. Candida parapsilosis: from Genes to the Bedside.

5. Activity of rabbit leukocyte peptides against Candida albicans.

6. Fungistatic and fungicidal activity of human parotid salivary histidine-rich polypeptides on Candida albicans.

7. Interaction of human defensins with Escherichia coli. Mechanism of bactericidal activity.

8. Inhibition of specific amino acid uptake in Candida albicans by lysosomal extracts from rabbit alveolar macrophages.

Review 9. Cutaneous defenses against dermatophytes and yeasts.

10. Antibody-dependent cell-mediated cytotoxicity against IBR-infected bovine kidney cells by ruminant neutrophils: the role of lysosomal cationic protein.