Identifying different types of de-differentiated microspores from indica-japonica F(1) hybrids with subspecies-differentiating RFLP probes in rice.

The indica, japonica and intermediary types of de-differentiated microspores from indica-japonica F(1) hybrids were identified with 11 subspecies-differentiating RELP probes in rice (Oryza sativa L.). The results showed that the distribution of indica, japonica and intermediary types of de-differentiated microspores could be easily detected in a simple and quick way using the RFLP method. Moreover, the microspores from the same F(1) hybrid but inoculated onto different media, or microspores from different F(1) hybrids when inoculated onto the same medium, often displayed distinctive distribution curves of de-differentiated microspores types, indicating that the media employed in this experiment had high selectivity for the de-differentiation of certain types of microspores. The application of the RELP method to de-differentiated microspore identification is of great theoretical and practical significance in rice doubled-haploid breeding.