Literature DB >> 19352020

Improvement of alkaliphily of Bacillus alkaline xylanase by introducing amino acid substitutions both on catalytic cleft and protein surface.

Hirohito Umemoto1, Mayuko Inami, Rie Yatsunami, Toshiaki Fukui, Takashi Kumasaka, Nobuo Tanaka, Satoshi Nakamura.   

Abstract

Xylanase J (XynJ) from alkaliphilic Bacillus sp. 41M-1 is an alkaline xylanase. The crystal structure has been solved with XynJ. Improvement of the alkaliphily of XynJ was attempted by amino acid substitutions. Reinforcing the characteristic salt bridge in the catalytic cleft and introducing excess Arg residues on the protein surface shifted the optimum pH of the wild-type enzyme from 8.5 to 9.5.

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Year:  2009        PMID： 19352020     DOI： 10.1271/bbb.80869

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  8 in total

1.  In silico rational design and systems engineering of disulfide bridges in the catalytic domain of an alkaline α-amylase from Alkalimonas amylolytica to improve thermostability.

Authors:  Long Liu; Zhuangmei Deng; Haiquan Yang; Jianghua Li; Hyun-dong Shin; Rachel R Chen; Guocheng Du; Jian Chen
Journal:  Appl Environ Microbiol       Date:  2013-11-08       Impact factor: 4.792

2.  Improvement of alkali stability and thermostability of Paenibacillus campinasensis Family-11 xylanase by directed evolution and site-directed mutagenesis.

Authors:  Hongchen Zheng; Yihan Liu; Mingzhe Sun; Yang Han; Jianling Wang; Junshe Sun; Fuping Lu
Journal:  J Ind Microbiol Biotechnol       Date:  2013-11-09       Impact factor: 3.346

3.  Cloning of a Family 11 Xylanase Gene from Bacillus amyloliquefaciens CH51 Isolated from Cheonggukjang.

Authors:  C U Baek; S G Lee; Y R Chung; I Cho; J H Kim
Journal:  Indian J Microbiol       Date:  2012-03-25       Impact factor: 2.461

4.  Roles of subunit NuoK (ND4L) in the energy-transducing mechanism of Escherichia coli NDH-1 (NADH:quinone oxidoreductase).

Authors:  Jesus Torres-Bacete; Prem Kumar Sinha; Motoaki Sato; Gaurav Patki; Mou-Chieh Kao; Akemi Matsuno-Yagi; Takao Yagi
Journal:  J Biol Chem       Date:  2012-10-27       Impact factor: 5.157

5.  Structural Insight into and Mutational Analysis of Family 11 Xylanases: Implications for Mechanisms of Higher pH Catalytic Adaptation.

Authors:  Wenqin Bai; Cheng Zhou; Yueju Zhao; Qinhong Wang; Yanhe Ma
Journal:  PLoS One       Date:  2015-07-10       Impact factor: 3.240

6.  Rational Design of Disulfide Bonds Increases Thermostability of a Mesophilic 1,3-1,4-β-Glucanase from Bacillus terquilensis.

Authors:  Chengtuo Niu; Linjiang Zhu; Xin Xu; Qi Li
Journal:  PLoS One       Date:  2016-04-21       Impact factor: 3.240

7.  A Novel Multi-domain High Molecular, Salt-Stable Alkaline Xylanase from Alkalibacterium sp. SL3.

Authors:  Guozeng Wang; Jingjing Wu; Renxiang Yan; Juan Lin; Xiuyun Ye
Journal:  Front Microbiol       Date:  2017-01-04       Impact factor: 5.640

8.  Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp. SN5 by random mutation and Glu135 saturation mutagenesis.

Authors:  Wenqin Bai; Yufan Cao; Jun Liu; Qinhong Wang; Zhenhu Jia
Journal:  BMC Biotechnol       Date:  2016-11-08       Impact factor: 2.563
  8 in total

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