Literature DB >> 19350081

Controlling the selection stringency of phage display using a microfluidic device.

Yanli Liu1, Jonathan D Adams, Kelisha Turner, Frank V Cochran, Sanjiv Sam Gambhir, H Tom Soh.   

Abstract

We report the utilization of microfluidic technology to phage selection and demonstrate that accurate control of washing stringency in our microfluidic magnetic separator (MMS) directly impacts the diversity of isolated peptide sequences. Reproducible generation of magnetic and fluidic forces allows controlled washing conditions that enable rapid convergence of selected peptide sequences. These findings may provide a foundation for the development of automated microsystems for rapid in vitro directed evolution of affinity reagents.

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Year:  2009        PMID： 19350081     DOI： 10.1039/b820985e

Source DB:  PubMed          Journal:  Lab Chip        ISSN： 1473-0189            Impact factor:   6.799


  24 in total

1.  Quantitative selection of DNA aptamers through microfluidic selection and high-throughput sequencing.

Authors:  Minseon Cho; Yi Xiao; Jeff Nie; Ron Stewart; Andrew T Csordas; Seung Soo Oh; James A Thomson; H Tom Soh
Journal:  Proc Natl Acad Sci U S A       Date:  2010-08-12       Impact factor: 11.205

Review 2.  Selection strategies for anticancer antibody discovery: searching off the beaten path.

Authors:  David Sánchez-Martín; Morten Dræby Sørensen; Simon Lykkemark; Laura Sanz; Peter Kristensen; Erkki Ruoslahti; Luis Álvarez-Vallina
Journal:  Trends Biotechnol       Date:  2015-03-26       Impact factor: 19.536

3.  Genetic analysis of H1N1 influenza virus from throat swab samples in a microfluidic system for point-of-care diagnostics.

Authors:  B Scott Ferguson; Steven F Buchsbaum; Ting-Ting Wu; Kuangwen Hsieh; Yi Xiao; Ren Sun; H Tom Soh
Journal:  J Am Chem Soc       Date:  2011-05-24       Impact factor: 15.419

4.  Integration of binding peptide selection and multifunctional particles as tool-box for capture of soluble proteins in serum.

Authors:  Angela Maria Cusano; Filippo Causa; Raffaella Della Moglie; Nunzia Falco; Pasqualina Liana Scognamiglio; Anna Aliberti; Raffaele Vecchione; Edmondo Battista; Daniela Marasco; Marika Savarese; Umberto Raucci; Nadia Rega; Paolo Antonio Netti
Journal:  J R Soc Interface       Date:  2014-10-06       Impact factor: 4.118

5.  Selection of phage-displayed peptides on live adherent cells in microfluidic channels.

Authors:  Jinpeng Wang; Yanli Liu; Tambet Teesalu; Kazuki N Sugahara; Venkata Ramana Kotamrajua; Jonathan D Adams; Brian S Ferguson; Qiang Gong; Seung Soo Oh; Andrew T Csordas; Minseon Cho; Erkki Ruoslahti; Yi Xiao; H Tom Soh
Journal:  Proc Natl Acad Sci U S A       Date:  2011-04-12       Impact factor: 11.205

6.  Rapid mRNA-display selection of an IL-6 inhibitor using continuous-flow magnetic separation.

Authors:  C Anders Olson; Jonathan D Adams; Terry T Takahashi; Hangfei Qi; Shannon M Howell; Ting-Ting Wu; Richard W Roberts; Ren Sun; H Tom Soh
Journal:  Angew Chem Int Ed Engl       Date:  2011-07-14       Impact factor: 15.336

Review 7.  Phage display in molecular imaging and diagnosis of cancer.

Authors:  Susan L Deutscher
Journal:  Chem Rev       Date:  2010-05-12       Impact factor: 60.622

8.  Improving aptamer selection efficiency through volume dilution, magnetic concentration, and continuous washing in microfluidic channels.

Authors:  Seung Soo Oh; Kareem M Ahmad; Minseon Cho; Seon Kim; Yi Xiao; H Tom Soh
Journal:  Anal Chem       Date:  2011-08-03       Impact factor: 6.986

Review 9.  Virus-Derived Peptides for Clinical Applications.

Authors:  Mingying Yang; Kegan Sunderland; Chuanbin Mao
Journal:  Chem Rev       Date:  2017-07-19       Impact factor: 60.622

10.  An integrated microfluidic system for screening of phage-displayed peptides specific to colon cancer cells and colon cancer stem cells.

Authors:  Yu-Jui Che; Huei-Wen Wu; Lien-Yu Hung; Ching-Ann Liu; Hwan-You Chang; Kuan Wang; Gwo-Bin Lee
Journal:  Biomicrofluidics       Date:  2015-10-15       Impact factor: 2.800
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