| Literature DB >> 19348731 |
L Zhao1, X Wei, Z Ma, D Feng, P Tu, B H Johnstone, K L March, Y Du.
Abstract
The delivery of factors secreted by adipose stromal cells (ASC) to the brain may be a viable neuroprotective therapeutic option. In this study, we investigated the bioactivity of ASC-conditioned medium (ASC-CM) in glutamate-induced neurotoxicity and found that the ASC-CM significantly blocked glutamate neurotoxicity. We identified the brain-derived neurotrophic factor (BDNF) in the ASC-CM by using Western blot and demonstrated that this activity was critical for the neuroprotective effect of ASC-CM in excytotoxicity models. Furthermore, inactivating BDNF also attenuated the suppression by ASC-CM of glutamate-induced caspase-3 activity, but not p38 phosphorylation. These findings suggest that among ASC secrete a potent combination of factors, BDNF play a major role in neuroprotection against excytotoxicity.Entities:
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Year: 2009 PMID： 19348731 DOI： 10.1016/j.neulet.2009.01.025
Source DB: PubMed Journal: Neurosci Lett ISSN： 0304-3940 Impact factor: 3.046