OBJECTIVES: Acute pancreatitis (AP) protease release induces lung parenchymal destruction via matrix metalloproteinases (MMPs), a neutrophil (polymorphonuclear leukocyte)-dependent process. Recent studies in hemorrhagic shock revealed that hypertonic saline (HTS) has an anti-inflammatory effect and can inhibit a variety of neutrophil functions. The aim of this study was to determine whether HTS and its actions in the pathway of neutrophil migration, MMPs, and heat shock proteins (HSPs) are effective in protecting the lung from injury associated with AP. METHODS: We determined neutrophil infiltration and expressions of MMPs and HSPs in the lung tissue after AP induced by retrograde infusion of 2.5% of sodium taurocholate. RESULTS: Animals submitted to AP that received HTS compared with those who received normal saline presented with increased HSP70 and HSP90 expressions and reduced myeloperoxidase levels and MMP-9 expression and activity. CONCLUSIONS: Our data raised the hypothesis that a sequence of HTS lung protection events increases HSP70 and HSP90, inhibiting infiltration of neutrophils and their protease actions in the lung.
OBJECTIVES: Acute pancreatitis (AP) protease release induces lung parenchymal destruction via matrix metalloproteinases (MMPs), a neutrophil (polymorphonuclear leukocyte)-dependent process. Recent studies in hemorrhagic shock revealed that hypertonic saline (HTS) has an anti-inflammatory effect and can inhibit a variety of neutrophil functions. The aim of this study was to determine whether HTS and its actions in the pathway of neutrophil migration, MMPs, and heat shock proteins (HSPs) are effective in protecting the lung from injury associated with AP. METHODS: We determined neutrophil infiltration and expressions of MMPs and HSPs in the lung tissue after AP induced by retrograde infusion of 2.5% of sodium taurocholate. RESULTS: Animals submitted to AP that received HTS compared with those who received normal saline presented with increased HSP70 and HSP90 expressions and reduced myeloperoxidase levels and MMP-9 expression and activity. CONCLUSIONS: Our data raised the hypothesis that a sequence of HTS lung protection events increases HSP70 and HSP90, inhibiting infiltration of neutrophils and their protease actions in the lung.
Authors: Ahsan U Shah; Amna Sarwar; Abrahim I Orabi; Samir Gautam; Wayne M Grant; Alexander J Park; Adnan U Shah; Jun Liu; Pramod K Mistry; Dhanpat Jain; Sohail Z Husain Journal: Am J Physiol Gastrointest Liver Physiol Date: 2009-11 Impact factor: 4.052
Authors: Tianming Le; John F Eisses; Kathryn L Lemon; John A Ozolek; Derek A Pociask; Abrahim I Orabi; Sohail Z Husain Journal: Pancreas Date: 2015-04 Impact factor: 3.327
Authors: Ricardo Garib; Priscila Garla; Raquel S Torrinhas; Ana I S Moretti; Marcel C C Machado; Dan L Waitzberg Journal: Mediators Inflamm Date: 2016-12-14 Impact factor: 4.711