Literature DB >> 19346357

Production of recombinant proteins in the lon-deficient BL21(DE3) strain of Escherichia coli in the absence of the DnaK chaperone.

Julien Ratelade1, Marie-Caroline Miot, Emmett Johnson, Jean-Michel Betton, Philippe Mazodier, Nadia Benaroudj.   

Abstract

To eliminate unavoidable contamination of purified recombinant proteins by DnaK, we present a unique approach employing a BL21(DE3) DeltadnaK strain of Escherichia coli. Selected representative purified proteins remained soluble, correctly assembled, and active. This finding establishes DnaK dispensability for protein production in BL21(DE3), which is void of Lon protease, key to eliminating unfolded proteins.

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Year:  2009        PMID： 19346357      PMCID： PMC2687262          DOI： 10.1128/AEM.00255-09

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  30 in total

1.  Sequential mechanism of solubilization and refolding of stable protein aggregates by a bichaperone network.

Authors:  P Goloubinoff; A Mogk; A P Zvi; T Tomoyasu; B Bukau
Journal:  Proc Natl Acad Sci U S A       Date:  1999-11-23       Impact factor: 11.205

2.  Protein aggregation in Escherichia coli: role of proteases.

Authors:  Ran Rosen; Dvora Biran; Eyal Gur; Dörte Becher; Michael Hecker; Eliora Z Ron
Journal:  FEMS Microbiol Lett       Date:  2002-01-22       Impact factor: 2.742

3.  Role of molecular chaperones in inclusion body formation.

Authors:  M Mar Carrió; Antonio Villaverde
Journal:  FEBS Lett       Date:  2003-02-27       Impact factor: 4.124

4.  ATP hydrolysis by the proteasome regulatory complex PAN serves multiple functions in protein degradation.

Authors:  Nadia Benaroudj; Peter Zwickl; Erika Seemüller; Wolfgang Baumeister; Alfred L Goldberg
Journal:  Mol Cell       Date:  2003-01       Impact factor: 17.970

5.  Isolation and characterization of a temperature-sensitive dnaK mutant of Escherichia coli B.

Authors:  H Itikawa; J Ryu
Journal:  J Bacteriol       Date:  1979-05       Impact factor: 3.490

6.  Locus for radiation resistance in Escherichia coli strain B-r.

Authors:  J Donch; Y S Chung; J Greenberg
Journal:  Genetics       Date:  1969-02       Impact factor: 4.562

7.  Recombinant maize protoporphyrinogen IX oxidase expressed in Escherichia coli forms complexes with GroEL and DnaK chaperones.

Authors:  A de Marco; S Volrath; T Bruyere; M Law; R Fonné-Pfister
Journal:  Protein Expr Purif       Date:  2000-10       Impact factor: 1.650

8.  NEMO trimerizes through its coiled-coil C-terminal domain.

Authors:  Fabrice Agou; Fei Ye; Stéphane Goffinont; Gilles Courtois; Shoji Yamaoka; Alain Israël; Michel Véron
Journal:  J Biol Chem       Date:  2002-03-04       Impact factor: 5.157

9.  Chaperone over-expression in Escherichia coli: apparent increased yields of soluble recombinant protein kinases are due mainly to soluble aggregates.

Authors:  Annette Haacke; Gabriele Fendrich; Paul Ramage; Martin Geiser
Journal:  Protein Expr Purif       Date:  2008-11-11       Impact factor: 1.650

10.  Removal of DnaK contamination during fusion protein purifications.

Authors:  Daniela V Rial; Eduardo A Ceccarelli
Journal:  Protein Expr Purif       Date:  2002-08       Impact factor: 1.650
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  15 in total

1.  Structural and functional characterization of an orphan ATP-binding cassette ATPase involved in manganese utilization and tolerance in Leptospira spp.

Authors:  Nadia Benaroudj; Frederick Saul; Jacques Bellalou; Isabelle Miras; Patrick Weber; Vincent Bondet; Gerald L Murray; Ben Adler; Paula Ristow; Hélène Louvel; Ahmed Haouz; Mathieu Picardeau
Journal:  J Bacteriol       Date:  2013-10-11       Impact factor: 3.490

Review 2.  A Review: Molecular Chaperone-mediated Folding, Unfolding and Disaggregation of Expressed Recombinant Proteins.

Authors:  Komal Fatima; Fatima Naqvi; Hooria Younas
Journal:  Cell Biochem Biophys       Date:  2021-02-25       Impact factor: 2.194

3.  A novel method for removing contaminant Hsp70 molecular chaperones from recombinant proteins.

Authors:  Enrique S Morales; Ivana L Parcerisa; Eduardo A Ceccarelli
Journal:  Protein Sci       Date:  2019-02-20       Impact factor: 6.725

Review 4.  The role of small molecules in musculoskeletal regeneration.

Authors:  Kevin W-H Lo; Keshia M Ashe; Ho Man Kan; Cato T Laurencin
Journal:  Regen Med       Date:  2012-07       Impact factor: 3.806

5.  Optimizing Chaperone Removal Strategy from Overexpressed Recombinant Proteins : GNE, a Case Study.

Authors:  Shweta Sharma; Roop Singh Bora; Kulvinder Singh Saini; Ranjana Arya
Journal:  Methods Mol Biol       Date:  2022

6.  Multi-omics Quantification of Species Variation of Escherichia coli Links Molecular Features with Strain Phenotypes.

Authors:  Jonathan M Monk; Anna Koza; Miguel A Campodonico; Daniel Machado; Jose Miguel Seoane; Bernhard O Palsson; Markus J Herrgård; Adam M Feist
Journal:  Cell Syst       Date:  2016-09-22       Impact factor: 10.304

7.  Enhanced fluorescent properties of an OmpT site deleted mutant of green fluorescent protein.

Authors:  Shardul S Salunkhe; Veena A Raiker; Sachin Rewanwar; Prakash Kotwal; Avijeet Kumar; Sriram Padmanabhan
Journal:  Microb Cell Fact       Date:  2010-04-29       Impact factor: 5.328

8.  Comparative transcriptomic profile analysis of fed-batch cultures expressing different recombinant proteins in Escherichia coli.

Authors:  Ashish K Sharma; Shubhashree Mahalik; Chaitali Ghosh; Anuradha B Singh; Krishna J Mukherjee
Journal:  AMB Express       Date:  2011-10-22       Impact factor: 3.298

9.  IcmF family protein TssM exhibits ATPase activity and energizes type VI secretion.

Authors:  Lay-Sun Ma; Franz Narberhaus; Erh-Min Lai
Journal:  J Biol Chem       Date:  2012-03-05       Impact factor: 5.157

10.  An Escherichia coli strain for expression of the connexin45 carboxyl terminus attached to the 4th transmembrane domain.

Authors:  Jennifer L Kopanic; Mona Al-Mugotir; Sydney Zach; Srustidhar Das; Rosslyn Grosely; Paul L Sorgen
Journal:  Front Pharmacol       Date:  2013-08-23       Impact factor: 5.810
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