Ancylobacter dichloromethanicus sp. nov.--a new aerobic facultatively methylotrophic bacterium utilizing dichloromethane.

A novel aerobic facultative methylotroph was isolated from contaminated soil. The organism (strain DM16) is a Gram-negative asporogenous non-motile curved rod multiplying by binary fission. Cells are neutrophilic and mesophilic. This strain utilized dichloromethane, methanol, formate and formaldehyde along with a variety of polycarbon compounds. Strain DM16 employs the ribulosebisphosphate pathway for C1 assimilation. The DNA G+C content is 64.5 mol%. The major ubiquinone is Q-10. The dominant cellular fatty acids are 18:1omega7c (58.6%), cyclo-19:0omega8c (34.8%) and 16:0 (3.2%). Sequencing of the 16S rRNA gene and DNA-DNA hybridization experiments clearly indicated that this methylotroph should be classified as a new species within genus Ancylobacter--Ancylobacter dichloromethanicus sp. nov. with the type strain DM16T (DSM 21507T = VKM B-2484T).