Literature DB >> 19344658

The development and evaluation of monoclonal antibodies for the detection of polycyclic aromatic hydrocarbons.

Candace R Spier1, Erin S Bromage, Thomas M Harris, Michael A Unger, Stephen L Kaattari.   

Abstract

A highly sensitive enzyme-linked immunosorbent assay (ELISA) for the detection of 3- to 5-ring polycyclic aromatic hydrocarbons (PAHs) has been developed. A functional derivative of dibenzothiophene was synthesized and covalently linked to carrier proteins that were used to produce monoclonal antibodies (mAbs). During the conjugation step, the conjugation efficiency was improved by the presence of 25% N,N-dimethylformamide (DMF). Antibodies were selected based on a competitive inhibition assay to isolate those with the highest sensitivity for free PAHs. When using the mAb in an ELISA format, free PAHs were detected at a concentration as low as 0.1 microg/L (0.1 ppb) in aqueous samples.

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Year:  2009        PMID： 19344658     DOI： 10.1016/j.ab.2009.01.020

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

1.  A highly sensitive monoclonal antibody based biosensor for quantifying 3-5 ring polycyclic aromatic hydrocarbons (PAHs) in aqueous environmental samples.

Authors:  Xin Li; Stephen L Kaattari; Mary A Vogelbein; George G Vadas; Michael A Unger
Journal:  Sens Biosensing Res       Date:  2016-03-01

2.  Biosensor applications in contaminated estuaries: Implications for disaster research response.

Authors:  Krisa Camargo; Mary Ann Vogelbein; Jennifer A Horney; Timothy M Dellapenna; Anthony H Knap; Jose L Sericano; Terry L Wade; Thomas J McDonald; Weihsueh A Chiu; Michael A Unger
Journal:  Environ Res       Date:  2021-08-19       Impact factor: 8.431

3.  Evaluation of a time efficient immunization strategy for anti-PAH antibody development.

Authors:  Xin Li; Stephen L Kaattari; Mary Ann Vogelbein; Michael A Unger
Journal:  J Immunoassay Immunochem       Date:  2016

4.  Investigating the quantitative structure-activity relationships for antibody recognition of two immunoassays for polycyclic aromatic hydrocarbons by multiple regression methods.

Authors:  Yan-Feng Zhang; Li Zhang; Zhi-Xian Gao; Shu-Gui Dai
Journal:  Sensors (Basel)       Date:  2012-07-09       Impact factor: 3.576

5.  Rapid fluorescence immunoassay of benzo[a]pyrene in mainstream cigarette smoke based on a dual-functional antibody-DNA conjugate.

Authors:  Ziyan Fan; Zhonghao Li; Shanshan Liu; Fei Yang; Zhaoyang Bian; Ying Wang; Gangling Tang; Qinxiao Zhao; Huimin Deng; Shili Liu
Journal:  RSC Adv       Date:  2018-08-21       Impact factor: 3.361

6.  A Rapid Extraction Method Combined with a Monoclonal Antibody-Based Immunoassay for the Detection of Amatoxins.

Authors:  Candace S Bever; Robert M Hnasko; Luisa W Cheng; Larry H Stanker
Journal:  Toxins (Basel)       Date:  2019-12-11       Impact factor: 4.546
  6 in total

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