| Literature DB >> 19343677 |
Clifford B Talbot1, James McGinty, David M Grant, Ewan J McGhee, Dylan M Owen, Wei Zhang, Tom D Bunney, Ian Munro, Beverly Isherwood, Rob Eagle, Andre Hargreaves, Chris Dunsby, Mark A A Neil, Paul M W French.
Abstract
We report an automated optically sectioning fluorescence lifetime imaging (FLIM) multiwell plate reader for high content analysis (HCA) in drug discovery and accelerated research in cell biology. The system utilizes a Nipkow disc confocal microscope and performs unsupervised FLIM with autofocus, automatic setting of acquisition parameters and automated localisation of cells in the field of view. We demonstrate its applications to test dye solutions, fixed and live cells and FLIM-FRET.Mesh：
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Year: 2008 PMID： 19343677 DOI： 10.1002/jbio.200810054
Source DB: PubMed Journal: J Biophotonics ISSN： 1864-063X Impact factor: 3.207