Literature DB >> 19343675

A compact, multidimensional spectrofluorometer exploiting supercontinuum generation.

Hugh B Manning1, Gordon T Kennedy, Dylan M Owen, David M Grant, Anthony I Magee, Mark A A Neil, Yoshifumi Itoh, Chris Dunsby, Paul M W French.   

Abstract

We report a novel, compact and automated multidimensional spectrofluorometer that exploits a fibre-laser-pumped ultrafast supercontinuum source to provide resolution with respect to intensity, excitation and emission wavelength, decay time and polarisation. This instrument has been applied to study the photophysics of the phase-sensitive membrane probe di-4-ANEPPDHQ and to characterise protein-protein interactions via Förster resonance energy transfer. It can be applied to in situ measurements via a fibre-optic probe in medical and other contexts and is demonstrated here to provide a comprehensive characterisation of tissue autofluorescence.

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Year:  2008        PMID： 19343675     DOI： 10.1002/jbio.200810051

Source DB:  PubMed          Journal:  J Biophotonics        ISSN： 1864-063X            Impact factor:   3.207


  16 in total

1.  Response of rigor cross-bridges to stretch detected by fluorescence lifetime imaging microscopy of myosin essential light chain in skeletal muscle fibers.

Authors:  Dmitry S Ushakov; Valentina Caorsi; Delisa Ibanez-Garcia; Hugh B Manning; Antonios D Konitsiotis; Timothy G West; Christopher Dunsby; Paul M French; Michael A Ferenczi
Journal:  J Biol Chem       Date:  2010-11-05       Impact factor: 5.157

2.  Membrane environment exerts an important influence on rac-mediated activation of phospholipase Cγ2.

Authors:  Katy L Everett; Anja Buehler; Tom D Bunney; Anca Margineanu; Rhona W Baxendale; Petra Vatter; Michael Retlich; Claudia Walliser; Hugh B Manning; Mark A A Neil; Christopher Dunsby; Paul M W French; Peter Gierschik; Matilda Katan
Journal:  Mol Cell Biol       Date:  2011-01-18       Impact factor: 4.272

3.  Application of time-resolved autofluorescence to label-free in vivo optical mapping of changes in tissue matrix and metabolism associated with myocardial infarction and heart failure.

Authors:  João Lagarto; Benjamin T Dyer; Clifford Talbot; Markus B Sikkel; Nicholas S Peters; Paul M W French; Alexander R Lyon; Chris Dunsby
Journal:  Biomed Opt Express       Date:  2015-01-07       Impact factor: 3.732

4.  Differential modes of DNA binding by mismatch uracil DNA glycosylase from Escherichia coli: implications for abasic lesion processing and enzyme communication in the base excision repair pathway.

Authors:  Seden Grippon; Qiyuan Zhao; Tom Robinson; Jacqueline J T Marshall; Rory J O'Neill; Hugh Manning; Gordon Kennedy; Christopher Dunsby; Mark Neil; Stephen E Halford; Paul M W French; Geoff S Baldwin
Journal:  Nucleic Acids Res       Date:  2010-11-25       Impact factor: 16.971

5.  In vivo fluorescence lifetime tomography of a FRET probe expressed in mouse.

Authors:  James McGinty; Daniel W Stuckey; Vadim Y Soloviev; Romain Laine; Marzena Wylezinska-Arridge; Dominic J Wells; Simon R Arridge; Paul M W French; Joseph V Hajnal; Alessandro Sardini
Journal:  Biomed Opt Express       Date:  2011-06-10       Impact factor: 3.732

6.  FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ.

Authors:  Sunil Kumar; Dominic Alibhai; Anca Margineanu; Romain Laine; Gordon Kennedy; James McGinty; Sean Warren; Douglas Kelly; Yuriy Alexandrov; Ian Munro; Clifford Talbot; Daniel W Stuckey; Christopher Kimberly; Bertrand Viellerobe; Francois Lacombe; Eric W-F Lam; Harriet Taylor; Margaret J Dallman; Gordon Stamp; Edward J Murray; Frank Stuhmeier; Alessandro Sardini; Matilda Katan; Daniel S Elson; Mark A A Neil; Chris Dunsby; Paul M W French
Journal:  Chemphyschem       Date:  2011-02-17       Impact factor: 3.102

7.  Correction Approach for Delta Function Convolution Model Fitting of Fluorescence Decay Data in the Case of a Monoexponential Reference Fluorophore.

Authors:  Clifford B Talbot; João Lagarto; Sean Warren; Mark A A Neil; Paul M W French; Chris Dunsby
Journal:  J Fluoresc       Date:  2015-06-11       Impact factor: 2.217

8.  A flexible wide-field FLIM endoscope utilising blue excitation light for label-free contrast of tissue.

Authors:  Hugh Sparks; Sean Warren; Joana Guedes; Nagisa Yoshida; Tze Choong Charn; Nadia Guerra; Taranjit Tatla; Christopher Dunsby; Paul French
Journal:  J Biophotonics       Date:  2014-02-27       Impact factor: 3.207

9.  Development of Low-Cost Instrumentation for Single Point Autofluorescence Lifetime Measurements.

Authors:  João Lagarto; Jonathan D Hares; Christopher Dunsby; Paul M W French
Journal:  J Fluoresc       Date:  2017-05-25       Impact factor: 2.217

10.  Fluorescence lifetime readouts of Troponin-C-based calcium FRET sensors: a quantitative comparison of CFP and mTFP1 as donor fluorophores.

Authors:  Romain Laine; Daniel W Stuckey; Hugh Manning; Sean C Warren; Gordon Kennedy; David Carling; Chris Dunsby; Alessandro Sardini; Paul M W French
Journal:  PLoS One       Date:  2012-11-09       Impact factor: 3.240
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