Literature DB >> 19342794

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of YvoA from Bacillus subtilis.

Marcus Resch1, Heide Marie Roth, Mathias Kottmair, Madhumati Sevvana, Ralph Bertram, Fritz Titgemeyer, Yves A Muller.   

Abstract

The putative transcriptional regulator protein YvoA (BSU35030) from Bacillus subtilis was cloned and heterologously expressed in Escherichia coli. The protein was purified by immobilized metal-affinity chromatography and size-exclusion chromatography and subsequently crystallized. A complete native data set was collected to 2.50 A resolution. The crystals belonged to the monoclinic space group C2 and preliminary analysis of the diffraction data indicated the presence of approximately 12 molecules per asymmetric unit.

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Year:  2009        PMID： 19342794      PMCID： PMC2664774          DOI： 10.1107/S1744309109008628

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  27 in total

1.  Subdivision of the helix-turn-helix GntR family of bacterial regulators in the FadR, HutC, MocR, and YtrA subfamilies.

Authors:  Sébastien Rigali; Adeline Derouaux; Fabrizio Giannotta; Jean Dusart
Journal:  J Biol Chem       Date:  2001-12-27       Impact factor: 5.157

2.  Crystal structure of FadR, a fatty acid-responsive transcription factor with a novel acyl coenzyme A-binding fold.

Authors:  D M van Aalten; C C DiRusso; J Knudsen; R K Wierenga
Journal:  EMBO J       Date:  2000-10-02       Impact factor: 11.598

3.  Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.

Authors:  J Reizer; S Bachem; A Reizer; M Arnaud; M H Saier; J Stülke
Journal:  Microbiology       Date:  1999-12       Impact factor: 2.777

4.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

Review 5.  Carbon catabolite repression in bacteria: choice of the carbon source and autoregulatory limitation of sugar utilization.

Authors:  Reinhold Brückner; Fritz Titgemeyer
Journal:  FEMS Microbiol Lett       Date:  2002-04-09       Impact factor: 2.742

6.  The three-dimensional structure of the N-acetylglucosamine-6-phosphate deacetylase, NagA, from Bacillus subtilis: a member of the urease superfamily.

Authors:  Florence Vincent; David Yates; Elspeth Garman; Gideon J Davies; James A Brannigan
Journal:  J Biol Chem       Date:  2003-10-13       Impact factor: 5.157

7.  Extending the classification of bacterial transcription factors beyond the helix-turn-helix motif as an alternative approach to discover new cis/trans relationships.

Authors:  Sébastien Rigali; Maximilian Schlicht; Paul Hoskisson; Harald Nothaft; Matthias Merzbacher; Bernard Joris; Fritz Titgemeyer
Journal:  Nucleic Acids Res       Date:  2004-06-24       Impact factor: 16.971

8.  PlmA, a new member of the GntR family, has plasmid maintenance functions in Anabaena sp. strain PCC 7120.

Authors:  Martin H Lee; Michael Scherer; Sébastien Rigali; James W Golden
Journal:  J Bacteriol       Date:  2003-08       Impact factor: 3.490

9.  Phosphoenolpyruvate phosphotransferase system and N-acetylglucosamine metabolism in Bacillus sphaericus.

Authors:  Alejandro F Alice; Gaspar Pérez-Martínez; Carmen Sánchez-Rivas
Journal:  Microbiology       Date:  2003-07       Impact factor: 2.777

10.  HutC/FarR-like bacterial transcription factors of the GntR family contain a small molecule-binding domain of the chorismate lyase fold.

Authors:  L Aravind; Vivek Anantharaman
Journal:  FEMS Microbiol Lett       Date:  2003-05-16       Impact factor: 2.742
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  2 in total

1.  Structural insight into operator dre-sites recognition and effector binding in the GntR/HutC transcription regulator NagR.

Authors:  Simon B Fillenberg; Florian C Grau; Gerald Seidel; Yves A Muller
Journal:  Nucleic Acids Res       Date:  2015-01-06       Impact factor: 16.971

2.  Insight into the induction mechanism of the GntR/HutC bacterial transcription regulator YvoA.

Authors:  Marcus Resch; Emile Schiltz; Fritz Titgemeyer; Yves A Muller
Journal:  Nucleic Acids Res       Date:  2010-01-04       Impact factor: 16.971
  2 in total

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