OBJECTIVE: We determined whether increasing adherence of multipotent stromal cells (MSCs) would amplify their effects on coronary collateral growth (CCG). METHODS AND RESULTS: Adhesion was established in cultured coronary endothelials cells (CECs) or MSCs treated with epidermal growth factor (EGF). EGF increased MSCs adhesion to CECs, and increased intercellular adhesion molecule (ICAM-1) or vascular cell adhesion molecule (VCAM-1) expression. Increased adherence was blocked by EGF receptor antagonism or antibodies to the adhesion molecules. To determine whether adherent MSCs, treated with EGF, would augment CCG, repetitive episodes of myocardial ischemia (RI) were introduced and CCG was measured from the ratio of collateral-dependent (CZ) and normal zone (NZ) flows. CZ/NZ was increased by MSCs without treatment versus RI-control and was further increased by EGF-treated MSCs. EGF-treated MSCs significantly improved myocardial function versus RI or RI+MSCs demonstrating that the increase in collateral flow was functionally significant. Engraftment of MSCs into myocardium was also increased by EGF treatment. CONCLUSIONS: These results reveal the importance of EGF in MSCs adhesion to endothelium and suggest that MSCs may be effective therapies for the stimulation of coronary collateral growth when interventions are used to increase their adhesion and homing (in vitro EGF treatment) to the jeopardized myocardium.
OBJECTIVE: We determined whether increasing adherence of multipotent stromal cells (MSCs) would amplify their effects on coronary collateral growth (CCG). METHODS AND RESULTS: Adhesion was established in cultured coronary endothelials cells (CECs) or MSCs treated with epidermal growth factor (EGF). EGF increased MSCs adhesion to CECs, and increased intercellular adhesion molecule (ICAM-1) or vascular cell adhesion molecule (VCAM-1) expression. Increased adherence was blocked by EGF receptor antagonism or antibodies to the adhesion molecules. To determine whether adherent MSCs, treated with EGF, would augment CCG, repetitive episodes of myocardial ischemia (RI) were introduced and CCG was measured from the ratio of collateral-dependent (CZ) and normal zone (NZ) flows. CZ/NZ was increased by MSCs without treatment versus RI-control and was further increased by EGF-treated MSCs. EGF-treated MSCs significantly improved myocardial function versus RI or RI+MSCs demonstrating that the increase in collateral flow was functionally significant. Engraftment of MSCs into myocardium was also increased by EGF treatment. CONCLUSIONS: These results reveal the importance of EGF in MSCs adhesion to endothelium and suggest that MSCs may be effective therapies for the stimulation of coronary collateral growth when interventions are used to increase their adhesion and homing (in vitro EGF treatment) to the jeopardized myocardium.
Authors: B M Arkonac; L C Foster; N E Sibinga; C Patterson; K Lai; J C Tsai; M E Lee; M A Perrella; E Haber Journal: J Biol Chem Date: 1998-02-20 Impact factor: 5.157
Authors: R Abramovitch; M Neeman; R Reich; I Stein; E Keshet; J Abraham; A Solomon; M Marikovsky Journal: FEBS Lett Date: 1998-04-03 Impact factor: 4.124
Authors: S Fujiyama; H Matsubara; Y Nozawa; K Maruyama; Y Mori; Y Tsutsumi; H Masaki; Y Uchiyama; Y Koyama; A Nose; O Iba; E Tateishi; N Ogata; N Jyo; S Higashiyama; T Iwasaka Journal: Circ Res Date: 2001-01-19 Impact factor: 17.367
Authors: M Simons; R O Bonow; N A Chronos; D J Cohen; F J Giordano; H K Hammond; R J Laham; W Li; M Pike; F W Sellke; T J Stegmann; J E Udelson; T K Rosengart Journal: Circulation Date: 2000-09-12 Impact factor: 29.690
Authors: D Orlic; J Kajstura; S Chimenti; I Jakoniuk; S M Anderson; B Li; J Pickel; R McKay; B Nadal-Ginard; D M Bodine; A Leri; P Anversa Journal: Nature Date: 2001-04-05 Impact factor: 49.962
Authors: Ali Amin; Soo-Kyoung Choi; Yehia Osman-Elazeik; Nariman K Badr El-Din; Christopher G Kevil; Louis G Navar; Philip Kadowitz; Mohamed Trebak; Khalid Matrougui Journal: Pflugers Arch Date: 2012-10-07 Impact factor: 3.657
Authors: Ali H Amin; Zakaria Y Abd Elmageed; Devika Nair; Megan I Partyka; Philip J Kadowitz; Souad Belmadani; Khalid Matrougui Journal: Lab Invest Date: 2010-05-03 Impact factor: 5.662
Authors: Meghan M Nickerson; Caitlin W Burke; Joshua K Meisner; Casey W Shuptrine; Ji Song; Richard J Price Journal: Angiogenesis Date: 2009-09-24 Impact factor: 9.596
Authors: Meghan M Nickerson; Ji Song; Joshua K Meisner; Sameer Bajikar; Caitlin W Burke; Casey W Shuptrine; Gary K Owens; Thomas C Skalak; Richard J Price Journal: Arterioscler Thromb Vasc Biol Date: 2009-09-04 Impact factor: 8.311