| Literature DB >> 19341768 |
Ratana Lawung1, Rungrot Cherdtrakulkiat, Angkana Charoenwatanachokchai, Sunanta Nabu, Wanvisa Suksaluk, Virapong Prachayasittikul.
Abstract
One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty Neisseria gonorrhoeae isolates, the penicillinase-producing N. gonorrhoeae (PPNG), the high-level tetracycline-resistant N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant N. gonorrhoeae (CRNG) were successfully classified. Our method provides expediency and benefit to epidemiology and antimicrobial-resistance mobility with 100% sensitivity and specificity for gonococcal-detection. The detection limit was 500 CFU/reaction.Entities:
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Year: 2009 PMID: 19341768 DOI: 10.1016/j.mimet.2009.03.009
Source DB: PubMed Journal: J Microbiol Methods ISSN: 0167-7012 Impact factor: 2.363