OBJECTIVE: To study the relationship between eotaxin and interleukin-4 (IL-4) in the pathophysiology of endometriosis. DESIGN: Comparative and laboratory study. SETTING: University teaching hospital reproductive endocrinology and infertility practice. PATIENT(S): Ectopic endometrial tissues were collected from women with endometriosis. INTERVENTION(S): Ectopic endometrial stromal cells (ESCs) were isolated and cultured with IL-4. Ectopic endometriotic tissues were immunostained for eotaxin and IL-4. MAIN OUTCOME MEASURE(S): Gene expression of eotaxin was determined by standard and real-time reverse-transcriptase polymerase chain reaction. Secretion of eotaxin from ESC was measured using specific ELISA. The immunostained sections were examined. RESULT(S): Interleukin-4 (IL-4) increased mRNA expression and protein secretion of eotaxin from ESC in a dose-dependent manner. Immunohistochemical analysis showed that eotaxin-positive cells colocalized with IL-4-positive cells and accumulated around the blood vessels in the stroma of endometriotic tissue. CONCLUSION(S): IL-4 induces eotaxin in ESCs, which might promote angiogenesis and the subsequent development of endometriosis. Copyright (c) 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
OBJECTIVE: To study the relationship between eotaxin and interleukin-4 (IL-4) in the pathophysiology of endometriosis. DESIGN: Comparative and laboratory study. SETTING: University teaching hospital reproductive endocrinology and infertility practice. PATIENT(S): Ectopic endometrial tissues were collected from women with endometriosis. INTERVENTION(S): Ectopic endometrial stromal cells (ESCs) were isolated and cultured with IL-4. Ectopic endometriotic tissues were immunostained for eotaxin and IL-4. MAIN OUTCOME MEASURE(S): Gene expression of eotaxin was determined by standard and real-time reverse-transcriptase polymerase chain reaction. Secretion of eotaxin from ESC was measured using specific ELISA. The immunostained sections were examined. RESULT(S): Interleukin-4 (IL-4) increased mRNA expression and protein secretion of eotaxin from ESC in a dose-dependent manner. Immunohistochemical analysis showed that eotaxin-positive cells colocalized with IL-4-positive cells and accumulated around the blood vessels in the stroma of endometriotic tissue. CONCLUSION(S): IL-4 induces eotaxin in ESCs, which might promote angiogenesis and the subsequent development of endometriosis. Copyright (c) 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Authors: Rodolfo D Vicetti Miguel; Nirk E Quispe Calla; Darlene Dixon; Robert A Foster; Andrea Gambotto; Stephen D Pavelko; Luanne Hall-Stoodley; Thomas L Cherpes Journal: Proc Natl Acad Sci U S A Date: 2017-08-01 Impact factor: 11.205
Authors: Andrei Mihai Măluţan; Tudor Drugan; Răzvan Ciortea; Radu Florin Mocan-Hognogi; Carmen Bucuri; Maria Patricia Rada; Dan Mihu Journal: J Res Med Sci Date: 2015-07 Impact factor: 1.852